Abstract: Objective：To elucidate the effects of fullerene（C₆₀）on reverse transcription（RT）. Methods：Template mRNA was extracted from the liver of zebrafish，synthesised cDNA with M-MLV reverse transcriptase. Different concentrations of C₆₀ were added into the RT reaction solution，to study the impact of C₆₀ on M-MLV reverse transcriptase and RNA conformation respectively. Results：The results showed that 100 μg/mL of C₆₀ could significantly inhibit the RT reaction，and this effect was related to the C₆₀ concentration. The RT inhibition of C₆₀ could be reversed by increasing the amount of M-MLV reverse transcriptase in the RT reaction solution. After its co-incubation with RNA in 37 ℃ for 12 h without light，C₆₀ can specifically degraded 28S rRNA in the concentration-dependent manner. Conclusion：These results demonstrate that C₆₀ could not only inhibit the activity of M-MLV reverse transcriptase，it also has damaging effect on the RNA. C₆₀ may have potential genetics toxicity.