Abstract: Through the comparison of three different anode substrates （grassiness straw hydrolysate,glucose simulated wastewater, Co-substrate solution of glucose and grassiness straw hydrolysate）with the same COD concentration, the writer studied the effects of different anode substrates to electricity generation voltage, electricity generation time, power density, internal resistance, coulombic efficiency and substrate COD removal rate of MFC. The results showed that the Co-substrate solution of glucose and grassiness straw hydrolysate as MFC anode substrate could obtain higher voltage and stable electricity generation over a long period of time; the maximum power density was 380. 63mW/m2; it had the lowest resistance of 155. 97 Ω; the coulombic efficiency was 22. 64%; the substrate removal rate of COD was 83. 2%, the Co-substrate solution can be used as MFC anode substrate.

Key words: microbial fuel cell, anode substrate, internal resistance, power density, coulombic efficiency