Abstract: Objective To explore the potential mechanism of ″ Sanhua decoction″ treating constipation using network pharmacology. Methods The active ingredients of Sanhua decoction were screened out by TCMSP，and the drug ″ drug-ingredient″ network was constructed through Cytoscape 3.9.1 software. Genecards were used to retrieve relevant targets of constipation，and the String platform was used to analyze protein interactions， build PPI networks ，and explore potential protein functional modules in the network. The ″ drug-component- target″ ，the biological processes，and pathways involved were analyzed through the Metascape platform. Results The results showed that the main active ingredients of Sanhua decoction were naringenin，luteolin，aloe emodin，ammidin，and neohesperidin. The core targets were ATK1，TP53，IL-6，TNF，CASP3，etc. The biological pathway mainly acted on the PI3K-AKT signaling pathway， the AMPK signaling pathway，and the NF- κB pathway. ConclusionThis study preliminarily reveals the multi-component，multi- target，and multi-pathway mechanism of Sanhua decoction in treating constipation，which provides a basis for the clinical utilization and development of Sanhua decoction.

Key words: Sanhua decoction, constipation, network pharmacology, mechanism