Abstract: Objective To explore the main active ingredients and mechanism of ″ Taohong Siwu Decoction″ promoting fracture healing using network pharmacology. Methods Using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP） to search the active ingredients corresponding to all drugs in ″ Taohong Siwu Decoction″ （THSWT），and screen the corresponding targets of the active ingredients. GeneCards database platform was used to mine the targets related to fracture healing（FH），and the Venn diagram of the ″ drug-disease″ common target was constructed. Cytoscape 3.8.2 was used to draw the network regulation map of ″drug active component-target-disease″，and the protein-protein interaction（PPI）network was constructed with the help of String 11.5 to obtain the number of adjacent nodes of core genes in the PPI network. Then Cytoscape 3.8.2 was used for topology analysis of the PPI network，and finally，the core genes were obtained. Functional annotation of gene ontology（GO）and pathway enrichment analysis based on the Kyoto Encyclopedia of Genes and Genomes（KEGG）was taken using the R language package clusterProfiler to predict the mechanism of ″ Taohong Siwu Decoction″ on promoting fracture healing. Results 69 active components involving 5 345 targets were predicted in ″Taohong Siwu Decoction″. 2 383 targets for fracture healing were identified，and 140 common target genes were obtained. The PPI analysis was used to identify ALB，AKT1，VEGFA，MMP2，MMP9，CASP3，JUN，and MYC as the core targets for promoting fracture healing. Enrichment analysis included 2 258 GO items（P < 0. 05） and 151 KEGG signaling pathways （P < 0. 05）. The main enrichment pathways were PI3K ? Akt signaling pathway，AGE ? RAGE signaling pathway，IL ? 17 signaling pathway，etc. Conclusion ″Taohong Siwu Decoction″ can promote fracture healing through multiple targets and pathways, which provides a research direction to further reveal its mechanism of action, and the effectiveness of the targets can be further verified through in vivo and in vitro experiments.

Key words: Taohong Siwu decoction, fracture healing, network pharmacology, active ingredient, mechanism of action