Abstract: In this study，58 representative local soybean germplasm resources from Hubei，Guangdong，and other regions were used as research materials. Through field experiments and testing，the protein content，fat content，water content，and total protein-fat content of each variety were determined，and statistical analysis was carried out. The results showed that the maximum protein content in 58 soybean germplasm resources was 51. 19%，the minimum was 40. 68%， and the variation coefficient was 12. 57%； the maximum fat content was 19. 87%， the minimum was 13. 31%， and the variation coefficient was 15. 85%； the maximum total protein-fat content was 66. 77%， the minimum was 56. 84%，and the variation coefficient was 9. 05%. After screening and comparison，16 of 58 soybean accessions were finally selected as excellent resources. Among them，MD216 from Qianjiang，Hubei，can be used as a special protein variety.

Key words: soybean, protein content, fat content, total protein-fat content, landraces, germplasm resources