Abstract: Objective The aim of this study is to develop the fingerprints of the Jieduyihao（JDYH） with high performance liquid chromatographic （HPLC） analysis and to provide the basis for the quality control of JDYH. Methods The HPLC fingerprints were conducted with the Ultimate TM XBC18（250 mm × 4. 6 mm）column in gradient elution. The analyses of corydaline，tetrahydropalmatine，berberine and glycyrrhizic acid were conducted with the mobile phase consisted of acetonitrile-waterphosphoric acid，at column temperature 30 ℃，flow rate of 1. 0 mL/min and detective wavelengths of 280 nm. The analyses of ginsenoside Re and ginsenoside Rb1 were conducted with the mobile phase consisted of acetonitrile-water in gradient elution，at column temperature 30 ℃，flow rate of 1. 0 mL/min and detective wavelengths of 203 nm. Results The fingerprints of ten batches of JDYH decoction were established，which contained 19 common peaks，the similarities among different batches were 0. 998， with RSD < 1% . Conclusion The analyses of HPLC fingerprints have high sensitivity and good reproducibility，which can provide a basis for scientific evaluation and quality control of JDYH.

Key words: Jieduyihao（JDYH）, fingerprint, high performance liquid chromatography（HPLC）