Abstract: Chooses two disinfectant，CTP-5 and carbendazim designs seven concentration gradient between 0~20 000 mg/L，after mushroom culture material handling，Inoculates mush-room strains and cultures. Researches the sterilizing effect of CTP-5 by observing the growth of mush-room mycelium and the growth of bacteria. The results show that CTP-5 and carbendazim both have obvious disinfection effect to mushroom culture material，the valid disinfection concentration of CTP-5 and carbendazim are respectively 10 000 mg/L and 8 000 mg/L. compared with high temperature sterilization group，the growth of mushroom mycelium is slower in medicone group. Compared with carbendazim group，the difference of disinfection effect and mycelium growth is not obvious in CTP-5 group. CTP-5 is a kind of new disinfectant which is innoxious and noresidue，can substitute for carbendazim to complementary disinfection and pollution prevention in mushroom culture material.

Key words: mushroom, CTP-5, carbendazim, disinfection, cotton seed hulls culture medium