Abstract: To screen high yield and good quality Prunella vulgaris L. varieties，which could be suitable for cultivation at Qichun areas，yield and quality of three Prunella vulgaris L. varieties（Hubei Qichun，Q；Jiangxi Zhangshu，J；Henan Queshan，H）were evaluated with an orthogonal experiment，according to the same plot，three different sowing dates（B1，B2，B3，respectively）and different fertilization treatments（F0，F1，F2，respectively）. The results showed that：In F2 fertilization treatment，⑴ In the B1 period，wet weight，total effective number and dry grain of fruit ear of Q variety were higher than that of J and H varieties（P < 0. 05），and rosmarinic acid content of three varieties was consistent（more than 0. 36%），indicating that Q variety is better in the B1 period. ⑵ In the B2 period，wet weight，total effective number and dry grain of fruit ear of H variety were higher than that in the other two cultivars（Q and J）（P < 0. 05），and rosmarinic acid content of three varieties was consistent（0. 27%～0. 29%），indicating that H variety is better in the B2 period. ⑶ In the B3 period，all parameters of three varieties（Q，J，H）were no significant differences. The content of rosmarinic acid of the fertilization F1 and F0 in different sowing dates was lower than that of F2 fertilization. Comprehensive evaluation showed that the Q variety could be used as the preferred cultivation and planting variety，with sowing date in the B1 period and F2 fertilization treatment，and the H variety could be moderately developed in Qichun areas.

Key words: Prunella vulgaris L., standardized cultivation, orthogonal experiment, yield, rosmarinic acid