Journal of Jianghan University(Natural Science Edition) ›› 2018, Vol. 46 ›› Issue (4): 299-304.doi: 10.16389/j.cnki.cn42-1737/n.2018.04.002

Previous Articles     Next Articles

Preliminary Research of Common Bean Seedlings Induced Technology by Cotyledonary Node

CHEN Gao,DAI Xiangde,SHAO Jingjie,REN Sanguo,CHEN Chanyou*   

  1. School of Life Sciences;Hubei Province Engineering Research Center for Legume Plants,Jianghan University,Wuhan 430056,Hubei,China
  • Online:2018-08-28 Published:2018-08-31
  • Contact: CHEN Chanyou

Abstract: The effects of different concentrations of auxins IBA(indole butyric acid), NAA(naphthalene acetic acid)and cytokines 6-BA(6-benzyl adenine),KT(kinetin)on the induction of adventitious buds and roots were studied from the cotyledonary node of "Bean white rice" common bean,and the common bean′s tissue culture seedlings were cultivated and transplanted. The results showed that the optimum medium for buds induction was MS + 1. 0 mg/L IBA + 0. 5 mg/L 6-BA + 0. 1 mg/L KT,the cofficient of the bud differentiation was 2. 89. The optimum rooting medium was MS + 0. 1 mg/L IBA,the rooting rate was 100%. The common bean′s tissue culture seedlings can survive after transplanting.

Key words: common bean, tissue culture, cotyledonary node, regenerated seedlings, cultivation and transplantation

CLC Number: