Journal of Jianghan University(Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (6): 78-81.

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Analysis on Micronucleus Formation Induced by Kidney Bean Lectin

MOU Longmei,LIU Yizi ,LI Jianan*   

  1. Department of Biotechnology ,Schoolof Life Science,Jianghan University,Wuhan 430056,Hubei,China
  • Online:2014-12-25 Published:2014-12-26

Abstract: Objective To research the effect on micronucleus formation induced by lectin from varieties of kidney beans. Methods Giving the mice lectin of three kind of kidney beans by intraperitoneal injection for 6 days. Bone marrow cells were collected and microscope slides were prepared by Giemsa staining. Micronucleus ratio was analysed under a light microscope, then judged the mutagenic effect of different kidney bean lection. Results There was no significant difference between experiment group and negative control group, but positive group was obviously higher than experiment group and negative control group(P<0. 05) . Micronucleus ratio had a dose-effect relationship,but it was independent with gender.Conclusion The three levels of lectin of three kind of kidney beans have no effect on micronucleus formation of bone marrow cells in mice,they can be used for development of biological preparations.

Key words: kidneybean, lectin , micronucleustest, mutageniceffect

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