Journal of Jianghan University(Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (5): 80-86.

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Analysis and Application of Transferable SSR Molecular Markers from Cowpea（Vigna unguiculata）to Pea（Pisum sativum）

PAN Lei 1，2 ， XU Yafang 1，2， GUO Rui 1，2， LI Jianan 1，2， HU Zhihui 1，2， CHEN Chanyou*1，2   

  1. 1. Hubei Province Engineering Research Center for Legume Plant， Wuhan 430056， Hubei， China；2. School of Life Sciences， Jianghan University， Wuhan 430056， Hubei， China
  • Online:2014-10-25 Published:2014-11-11
  • Contact: CHEN Chanyou
  • About author:陈禅友 （1963—） ， 男， 教授， 博士， 研究方向： 植物遗传与育种。E-mail： ccy@ jhun.edu.cn

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Abstract: Aimed to screen out transferable SSR（simple sequence repeat）molecular markers from cowpea（Vigna unguiculata）to pea（Pisum sativum） . Using thirteen cowpea SSR primer pairs，eight primer pairs were successfully cross-amplified in pea. Five of the eight primer pairs exhibited polymorphism，and then to study the genetic variation of eleven pea cultivars and five cowpea cultivars. Results showed that genetic parameters of the average number of alleles，the average effective number of alleles and the average Shannon′s index are 2. 00，1. 64，0. 55 in the pea cultivar group，and 2. 00，1. 80，0. 53 in the cowpea cultivar group，respectively. These indicated that a moderate level of genetic diversity was revealed in the two cultivar groups. In addtion，UPGMA clustering showed that the pea cultivar group and the cowpea cultivar group clustered into their own branches，respectively. Within each group，most of the samples can be distinguished from each other. Especially，within the pea cultivar group，all the samples can be divided into two subgroups，which are consistent with their geographical origin.

Key words: Pisum sativum, Vigna unguiculata, simple sequence repeat(SSR), transferability

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