Abstract: Selected three kinds of bacteria which are most common in mushroom culture medium and are difficult to be killed（E. coli，Bacillus subtilis，Aspergillus）as the test object，to test the sterilization effect of sodium percarbonate and microwave. The sodium percarbonate had the concentration gradient as 20 000，10 000，1 000，100，50，10 mg/L. The experiments showed the amount of three bacteria reduced as the concentration of sodium percarbonate increased. The E. coli and the Aspergillus were all killed at the concentration of 10 000 mg/L，the Bacillus subtilis were all killed at the concentration of 20 000 mg/L. As the time of microwave increased，the amount of bacteria reduced，the Aspergillus were all killed at 210 s of microwave，the E. coli were all killed at 240 s of microwave，the Bacillus subtilis were all killed at 300 s of microwave，the experiment reached the complete sterilization.

Key words: sodium percarbonate, microwave, disinfection, sterilization, edible fungi culture medium