Journal of Jianghan University(Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (5): 389-392.doi: 10.16389/j.cnki.cn42-1737/n.2015.05.001

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Effects of Different Disinfectant on Cowpea Seeds′ Sterilization Culture

WU Hua1，3，GAO Zhipeng1，2，CHEN Gao1，2，SONG Lun1，2，CHEN Chanyou*1，2   

  1. 1. School of Life Sciences，Jianghan University，Wuhan 430056，Hubei，China；2. Hubei Province Engineering Research Center for Legume Plants，Wuhan 430056，Hubei，China；3. Cosmetics Department，School of Science，Beijing Technology and Business University，Beijing 100048，China
  • Online:2015-10-28 Published:2015-11-02
  • Contact: CHEN Chanyou
  • About author:陈禅友（1963—），男，教授，博士，研究方向：植物遗传学。E-mail：ccy@jhun.edu.cn

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Abstract: The effects of different seed disinfection methods on Longwen cultivar cowpea seeds were investigated. Optimum results were achieved by surface sterilizing the seeds with 0. 1% mercury bichloride solution for 8 minutes（contamination rate 11%，germination rate 77%）. The contamination rate can be efficiently decreased by adding 300 mg/L cefotaxime sodium to the medium after surface sterilization（7%）. This method does not affect the normal growth of plants，85% of plants reached the height of 3 cm after 10 days.

Key words: cowpea, disinfectant, mercury bichloride, sodium hypochlorite, cefotaxime sodium, aseptic culture

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