Table of Content

20 October 2012, Volume 40 Issue 5

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Orignal Article

Several Conclusions about the Erlang(2) Risk Model

GAO Ping

2012, 40(5):  11-15. 

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Consideres a diffusion perturbed Erlang(2) risk model with a constant dividend barrier, discusses the integro－differential equation with certain boundary conditions for M(x,y;b), the integral equation for M(x,y;b), obtaines the conditions under which M(x,y;b) is continuously differentiable through the integral equation.

Fault Diagnosis of Low Voltage Electrical EquipmentBased on Rough Set Theory

LIU Ya－li,HUANG Xun

2012, 40(5):  16-18. 

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According to fault analysis of low voltage electrical equipment, proposed a fault diagnosis method based on rough set theory. Basis of the fault records, the condition attributes set and decision attributes set is established. The condition attributes set is simplified and the corresponding rule set is acquired. Finally, making fault diagnosis for low voltage electrical equipment by use of the rule set.

Light Squeezing of the Non－resonant Interaction System Between Two－atomes and Field in Dissipative Cavity

ZHANG Li－hui,SHI Wen－xing

2012, 40(5):  19-22. 

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By means of numerical calculations, the squeezing property of the light field in the non－resonant system of two entangled atoms interacting with coherent field in dissipative cavity is studied. The effects of the detuning between the atom and cavity field, the initial field intensity, the decay of the cavity field on the light squeezing are discussed. The results show that when two entangled atoms interact with coherent field in the non－resonat system, light squeezing may occur, the squeezing degree has the periodicity with the time evolution. Also, the squeezing degree of light field becomes strong, the squeezing time increases, when the initial field intensity is adjusted appropriately. With the increase of the decay of the cavity field, the squeezing degree of light field gradually becomes weak, the squeezing time gradually decreases.

Study of Highly Accurate Signal Frequency Measurement

LEI Hai－dong

2012, 40(5):  23-26. 

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Frequency measurement is an assessment method of the frequency source stability in the time field. The highly accurate measurement is realized by using the DDS module, counter module and precision time measurement module. The principle and characteristics of the complete instrument are described.

Experiment of Cracking of Tar Model Compound Toluene Under Microwave

MI Tie,YU Xin－ming,WU Zheng-shun

2012, 40(5):  27-31. 

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Using different heating carrier C, Al2O3 and adding catalytic material Ni on the carriers, research the cracking of tar model compound toluene with the materials under microwave heating and ordinary oven, investigates the influence of heating method, catalytic component, heat carrier to cracking of toluene. The results show that there is no obvious influence to toluene cracking with microware heating or with ordinary heating, but microwave heating has certain inhibiting function on carbon build-up; when the inlet concentration of toluene is 4.86-5.80 g/Nm3, space-time is 0.124-0.125 s, towards different bed material, the microwave heating cracking rate is 26.24%-55%; and to ordinary heating, under 650℃, the ordinary heating cracking rate with the same bed material is 66.02%-100%; catalytic activity center-Ni has obvious catalytic seletivity on the carrier; meanwhile the crack of toluene, there is small sum of toluene transforming to high aromatic series degree.

Enhancing Nitric Oxide Removel from Simulated Flue Gas with Ultrasound

WANG Fang,LI Xiao－lu,YU Guo－xian,JIN Mei,LU Ping

2012, 40(5):  32-36. 

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A mixture of N2 and NO was used as the simulated flue gas, and removal of NO with using the acid urea solution as the sorbent was studied. Investigated the effects of concentration of NO,gas flow rate,concentration of absorbent, pH value of absorption solution,temperature of absorption solution on removal rate. NO removel experiment was conducted in ultrasonic environment. The results showed that the removal rate of NO could reach 86.84 ％ under the optimum condition as followsthe temperature was 50 ℃, the NO initial concentration was3 300 mg／m3, the gas flow rate was 300 mL／min, the urea mass fraction was 10 ％, the pH was 3.00. The ultrasonic played certain role in promoting the absorption process. The experiment indicated that when the ultrasonic power was 5 kW, the removel rate of NO could reach 89.13％.

Synthesis of Coumarins -3- ethyl—Earboxylate Catalyzed with Chitosan

PENG Wang-ming

2012, 40(5):  37-40. 

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Coumarin－3－ ethyl—carboxylate was synthesized with salicylaldehyde and diethylmalonate by using absolute alcohol as solvent and chitosan as catalyst. Some factors that influnced the yield of coumarin－3－ ethyl—carboxylate were studied. The results showed that the yield of coumarin－3－ ethyl—carboxylate was up to 87％ when diethylmalonate was 0.06 mol, salicylaldehyde was 0.05 mol, chitosan was 3.0 g, the reaction time was 120 min, and absolute alcohol was 10 mL, the temperature was 70 ℃.

Blocking Effect of Ginkgo Leaf to Nitrosamin Synthesis

GUO Yan－huaa,ZHANG Yu－mina,XU Guo－quanb

2012, 40(5):  41-44. 

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Compares the effect to block nitrosamin synthesis of extraction of ginkgo leaf with different extractant under the conditions that the solid-to-liquid ratio is 1∶10, extracting temperature is 80℃, extracting time is 60 mins, studies the blocking effect to nitrosamin synthesis and clearance effect to sodium nitrite of ginkgo leaf active material with different concentration extracted by 55％ acetone. The results show the suitable extractant is 55％ acetone, the extraction has relative high function to block nitrosamin synthesis and to clear sodium nitrite. When the solid content is 43.60 mg／mL, the blocking ability to nitrosamin systhesis of ginkgo leaf is 73.02％, and the clearance rate to sodium nitrite is 75.92％.

Histogram Processing in Image Spatial Domain Enhancing Processing

LIU De-quan^,

2012, 40(5):  45-48. 

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The transform enhancement of image grayscale is an direct image processing method based on spatial domain in image enchancing processing technology, using histogram to show gray series in gray image is researched. On matlab platform, through programming, researches the equalization and specification algorithm of histogram, which makes the histogram homogeneous distribute and enchances the image.

Signal Control and Simulation of Intersections Based on Bus Priority

LIU Xia,GUO Zhen,PEI Cong

2012, 40(5):  49-52. 

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Based on investigation of traffic flow and current signal timing of intersections, the green ratio is determined by passenger flow ratio and phase saturation so as to minimize per capita delay. Signal timing is optimized and bus trip efficiency is improved. Instances are tested by theoretical calculation and software simulation, the results show that when the phase flow is not saturated, the green ratio optimization method can improve the entrance lane capacity utilization rate, reduce average passenger delay and implement bus priority on the premise of traffic smoothing.

Color Clustering on RGB Images Based on K－means Algorithm

HAN Hai

2012, 40(5):  53-55. 

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Gives a algorithm which uses K－means algorithm to iterate and cluster, and uses the clustering results to build up color image palette. The algorithm based on the statistic of the appearing times of all colors RGB combination value, uses the 256 color from cluster to build up palette, and tranforms the BMP format-pattern into GIF formate. The experiments show that the transform has less color distortion.

html.Design and Realization of Server－side JavaScript ContainerBased on Rhino Engine

LI Yi

2012, 40(5):  56-60. 

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Nowadays JavaScript is re－valued, and its running environment is shifting gradually from traditional web client－side to server－side. As a typical representative of server－side JavaScript engine, Rhino has a broad platform support and appling foundation. By placing Rhino engine into a standard Java servlet, a server－side JavaScript container can be constructed. With the help of Rhino engine, developers can not only access Java objects inside JavaScript container, but also gain full support of Java platform. Meanwhile, server－side JavaScript also unified development language used in both front and back end. Thus, it reduces the difficulty of web development and improves efficiency.

Analysis for Dynamic Load Effect of Chimney Blasting Demolition

LIU Zi－qiang,GONG Xiang－chao,XIAO Meng－li,WANG Yi

2012, 40(5):  61-63. 

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In the Chimney blasting demolition, field data shows that chimney is affected by the dynamic load effect in blasting instant. If the blasting cut angle is designed improper, it will affect the accuracy of the chimney dumping direction. Relies on the law of conservation of energy, the computational formula of the impact dynamic factor of chimney blasting is deduced, the dynamic stress of the remaining weak cross－section of the chimney blasting cut is studied .By theory calculating the value of the stress of the remaining part under the action of blasting impact load, comes to the higher limit of the cut angle which ensuring that the chimney would not sitting down. Engineering examples and field data are given to verify the correctness of the theory.

Prokaryotic Expression and Functional Characterization of Scorpion Toxins Gene BmKbpp from Buthus Martensii Karsch

LUO Feng,LI Zheng,FAN Li－mei,LIANG Yong,LIN Yan,JIANG Lin

2012, 40(5):  64-70. 

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Primary structure analyzing and secondary structure predicting indicate that BmKBPP of NDBPs (Non－Disulfide－Bridged Peptides) might be a polycovalent cationic host defense peptide, which has a typical structure of amphiphilic α－helix and plenty of basic amino acids(Arg and Lys)with net positive charges. Using PCR method and plasmid vector pGEX－5X－1, the recombinant expression vectors of BmKbpp, EGFP and BmKbpp／EGFP prokaryoticly fused with GST were constructed. These three expression vectors and pGEX－5X－1 were transformed into Rossetta(DE3) strains, respectively. Expression of BmKbpp gene in E. coli demonstrates that its product has antimicrobial function by testing the living curve of host cell. Although the fusing products of genes is toxic to host cell, the slight fusion expression of GST, BmKBPP and EGFP is successful, which provides us a way to obtain BmKBPP for further functional research.

PCR Efficiency of Short Repetitive Sequences (BmKb1)n in Tandem Expression Vector and Optimization of Its PCR Conditions

FAN Li－mei,LI Zheng,YU Teng,LIU Yan－qun,LUO Feng

2012, 40(5):  71-75. 

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After acquiring BmKb1 gene by Overlap PCR, pGEX－6p－1／BmKb1 vector was constructed. The series recombinant pGEX－6p－1／(BmKb1)n (n ＝ 2, 4, 6, 8, 10) plasmids were build with isocaudamer technology. With the template of pGEX－6p－1／(BmKb1)n, standard PCR procedure was used to study PCR efficiency of the short tandem repeats; The BmKb1 octoploid template was used to study the effects of the number of PCR cycles, the final concentration of primers and the annealing temperature on PCR efficiency and specificity of the short sequence repeat, respectively. The results showed thatwith the number of tandem repeat sequences increasing, the number of non－specific bands will increase, and the content of specific bands will decrease; Amplifying BmKb1 gene tandem by PCR in the 18-20 cycle numbers, with the primer final concentration of 0.05-0.1 μM, as well as at higher annealing temperature of 60-62 ℃, the relatively high yield and high the specificity PCR fragments of BmKb1 gene concatemer will be available. This study will provide a technology basis for the cloning, PCR screening, sequencing of tandem expression vector and genome STRs.

Deletion of Efflux Pump Gene acrAB in E. coli withPCR Product One-step Method

JI Fang,YAN Zhi-xiong,LI Wen-dan,ZHU He,LIANG Yong

2012, 40(5):  76-79. 

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A PCR fragment including 30nt of flanking DNA homologous to the chromosome in each end and a antibiotic－resistant gene in the middle is introduced into the host cell of BW25113／ pIJ790. The three proteins of λ phage－－－Exo、Bet and Gam, expressed by pIJ790, composing the Red recombination system has the advantage of high one－step recombination rate of linear fragment. The antibiotic－resistant gene replaces the target gene as a result. This research obtaines a derivative of E. coli whose efflux pump gene acrAB is deleted. The mutant strain will help to research of mechanism of antimicrobial materials.

Combined Effect Study of C60／Cu2＋ Affecting Plasmid DNA Conformation

LI Jia,LIN Yan,LI Zhun－jie,LI Zheng,LIANG Yong

2012, 40(5):  80-84. 

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The toxic effects of carbon nanoparticles have been increasingly gaining attention. In this research, the combined effect of C60／Cu2＋ on DNA conformation was comparatively studied by altering various reaction conditions, to assess the biological toxicity of carbon nanoparticles. The results showed that without photoexcitation, C60／Cu2＋ could significantly induce the conversion of supercoiled DNA into nicked DNA, and this DNA cleavage effect was enhanced as the concentration of Cu2＋ and C60 increased. Moreover, the cleaved DNA could be reconnected to circular DNA by T4 DNA ligase, which indicated that the phosphodiester bond was the splicing site of C60／Cu2＋. In addition, various ROS savengers could not reduce the DNA cleavage effect of C60／Cu2＋, which was relate to the feature that Cu2＋ played a pivotal role in DNA cleavage. Taken together, these results demonstrated that C60／Cu2＋ could significantly induce the DNA cleavage without photoexcitation, which exhibited obvious combined effect. Further consideration should be given in assessment of their potential genotoxicity.

Extracting Condition of Extraction of Eleocharis Tuberosa Peel

XU Guo－quana,GUO Yan－huab,RAN Ya－chia,MEI Lia,YU Zhena

2012, 40(5):  85-89. 

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Uses different extractant to heat and extract eleocharis tuberosa peel powder, optimizes the best extractant, on the basis of single factor experiment, orthogonal test is used to optimize the technological conditions; meanwhile, discusses the extracting effect of peel of different particle size and different production place. The results show that the extraction reaches the highest level under the conditions that the extractant is 36％ acetic acid, material－solution ratio is 1∶12(g∶mL), extracting temperature is 50℃, extracting time is 4 hours, for 2 times extracting; the extraction is more as the peel powder is smaller; through the comparision of peel produced in Hubei and Guangxi, illustrates under different extracting condictions, the production place of peel has influence to extract.

Effect of TanshinoneⅡA Promoting Endothelial Cells Proliferation

TU Qian,YE Yong,YANG Dan-dan

2012, 40(5):  90-92. 

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ObjectiveTo study the effects of tanshinoneⅡA promoting endothelial cells proliferation and investigate the proper dose and action time. MethodsRespectively investigates the situation of cell proliferation with SRB after different concentration tanshinoneⅡA incubating the human umbilical vein cells (HUV-EC-C) for 24 h and 48 h. ResultsTanshinoneⅡA has the obvious effect to promote cell proliferation of human umbilical vein cells(HUV-EC-C), the effect is most obvious under the conditions that the concentration is 6 mg/L and the intervening time is 48 h. ConclusionTanshinoneⅡA can promote endothelial cell proliferation which play an important role on angiogenesis.

Correlated Analysis of Serum HBV－DNA Level and HBV Markersin 335 Cases of Hepatitis B Patients

CHEN Feng－ping,SHEN Yun－feng,WU Yao,ZHANG Hong－bo

2012, 40(5):  93-95. 

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ObjectiveTo study the association between HBV－DNA level and HBV markers in hepatitis B patients. MethodsA total of 335 hepatitis B patients were recruited in this study. Serum HBV－DNA level was quantified by fluorescence quantitative polymerase chain reaction(FQ－PCR)and serum HBV markers were detected by enzyme－linked immunosorbent assay(ELISA) separately. The association between HBV－DNA level and HBV markers was primarily investigated by statistical analysis. ResultsThe HBV－DNA positivity and level were significantly higher in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients than those in other groups(P ＜ 0. 01); The HBV－DNA positivity and level were significantly lower in HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients than those in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients(P ＜ 0. 01), but the differences were no statistical significance between HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients and HBsAg(＋)、HBcAb(＋) patients(P ＞ 0. 05); HBV－DNA positivity was significantly higher in HbeAg(＋) patients than in HbeAg(－) patients(P ＜ 0. 01). ConclusionCombining quantitative detection of HBV－DNA with HBV markers, in order to provide a reliable basis for judging the hepatitis B infection, virus replication, infectious intensity and the effect of antiviral observations.

Therapeutic Effect of Er Long Capsule on Sensorineural Hearing Loss Associated with Secretory Otitis Media

XU Xiang,XIAO Cai-wen,HE Qing-wen,LI Hui

2012, 40(5):  98-99. 

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ObjectiveTo investigate the therapeutic effect of Er Long capsule in the treatment on sensorineural hearing loss associated with secretory otitis media. MethodsRandomly divided 71 cases into two groupsnormal therapeutic methods adds Er Long capsule group(therapic group) and normal therapeutic methods control group(control group). ResultsIn the control group,the effective mate was 70％ while that was 86.3％ in the treatment group (P＜0.05). The treatment effect of Er Long group is better than control group, there is statistics significance(P＜0.05). ConclusionEr Long capsule is an effective medicine in the treatment on sensorineural hearing loss associated with secretory otitis media.

Application of Modern Medical Education Concept in Teaching ofMedical Microbiology

HU Song,SONG Wen-jian

2012, 40(5):  105-107. 

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With the development of science and technology, the modern medical education has played a great role in teaching of medical microbiology. Takes the student as the center, and attach great importance to the cultivation of students′ initiative, creativity and adaptability. Pays close attention to discipline integration and fusion. In class, it is important to pay special attention to experimental teaching and assessment. Humanistic education and technology education in the medical education are equally important. Focuses on a number of recent years public healthy event in education.

Application of PBL Teaching Method in Dental Hard Tissue Disease Comprehensive Treatment

ZHANG Biao,GAO Yang,XIA Xuan－tong,LIU Ping－xian

2012, 40(5):  108-111. 

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ObjectiveTo explore the teaching effect of comprehensive treatment to dental hard tissue disease, compares the problem based learning(PBL) method and lecture based learning(LBL) method. MethodsDivided the students into experimental group and control group, the experimental group had the PBL education while the control group had the LBL education, tested the two group students in the theoretical examination, cases analysis and operating skills, and compared the performance. ResultsThere are no stastical significance between two group students in theoretical examination and operating skills, but in cases analysis, the experimental group was obvious higher than control group. ConclusionLeads PBL teaching method into dental hard tissue comprehensive treatment can improve cases analysis ability of students.