Journal of Jianghan University(Natural Science Edition) ›› 2018, Vol. 46 ›› Issue (5): 438-445.doi: 10.16389/j.cnki.cn42-1737/n.2018.05.008

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Analysis of O-acetylation Modification on Sialylated N-glycans with Dual Modifications and UPLC-MS/MS

FU Xian，ZHANG Qiwei，WU Zhaoguan，ZHENG Qi*   

  1. School of Chemistry and Environmental Engineering；Institute for Interdisciplinary Research，Jianghan University，Wuhan 430056，Hubei，China
  • Online:2018-10-28 Published:2018-10-25
  • Contact: ZHENG Qi

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Abstract: The sialylated N-glycans from freshwater crucian carp serum are rich in O-acetylation modification. This article provided an in- depth analysis of the O- acetylation distribution in dual modifications sialylated N-glycans released from the crucian carp serum with UPLC-Orbitrap-MS/MS. Uneven distribution of O- acetylation was identified in sialylated N- glycans isomers. The results enriched the biological information of glycomics of crucian carp. The established method has a wide application prospect in the research of glycan modification and the search for glycoprotein biomarkers.

Key words: sialylated N-glycans, O-acetylation, isomers, dual modifications, UPLC-MS/MS

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