Abstract: Objective To investigate the different effect of Sorafenib and TRAIL on proliferation inhibition and apoptosis inducing of K562 cells. Methods Inhibition of K562 cells proliferation by Sorafenib alone and combined with TRAIL were studied in vitro using CCK-8. Apoptosis inducing rate of K562 cells by Sorafenib alone and combined with TRAIL were accessed by Western-Blot detection. Results Combination with Sorafenib and TRAIL synergistically increased the growth inhibition effects and enhanced the apoptosis rate of K562 cells. Conclusion Combination treatment of Sorafenib and TRAIL showed a synergistic anti-proliferative effect in K562 cells.