Abstract: Objective： To explore the distribution and antibiotic resistance of extended-spectrum-β-lactamases（ESBLs）-producing Escherichia coli in the hospital，so as to provide reference for reasonable utilization of antibiotic and reducing of drug resistance. Methods：From Aug，2010 to Dec，2011， 264 E.colis were isolated from clinical specimens，the antibiotic susceptibility was tested by K-B method. According to CLSI standard，ESBLs was confirmed，statistical analysis was taken with WHONET 5.4 software. Results：Among 264 E.coli isolates， 134 （50. 8%）were ESBLs-producing strains. The sputum was the highest sources of specimens（47. 76%），the department of general surgery was the highest section-office of distribution（12. 61%） . The resistance rates for ESBLs-producing E.coli to imipenem，meropenem，piperacillin/tazobactam，cefoperazone/sulbactam，amikacin，cefoxitin and nitrofurantoin were lower and between 0% to 15. 5%；The resistance rates to ciprofloxacin，levofloxacin， gentamicin，compound sulfamethoxazole and amoxicillin/clavulanate were higher and between 41. 3% to 82. 8%；The resistance rates to penicillins，cephalosporins and aztreonams were highest and reached 100%，so they are not suitable for clinic. Conclusion：ESBLs-producing E.coli has multidrug-resistant，so it is necessary to strengthen the surveillance in clinical laboratory，to use antibiotics according to antibiotic susceptibility test.