Abstract: Rutin combined iron complexes was synthesized with rutin and ferric sulfate at 40 ℃ and pH subacidic conditions. The composition of the complexes of Fe （Rutin）₂ ·5H₂O was determined with equimolar continuous variation and differential thermal analysis. Structure of the complexes were characterized by UV and IR. The maximum absorption of the composition was at 416 nm and Fe-O bond characteristic absorption peaks appeared at 620. 94 cm^-1. The interaction mechanism of the composition with DNA was studied by the methods of absorption spectrometry and fluorescence emission spectroscopy. The bonding constant of rutin- Fe （Ⅲ）to DNA was 67. 57 mL/mg. The experimental results indicated that the composition can bind to DNA in embedded mode.

Key words: rutin-Fe complexes, rutin, DNA bonding mode