Abstract: Constructed the fingerprint of 9 velvet bean（Stizolobium cochinchinensis）germplasm resources and analyse their genetic diversity with ISSR markers. The results indicated that 8 primers with strong stability and repeatability were screened from 35 primers and carried out PCR amplification. 64 bands were amplified from 9 velvet bean varieties，among which 50 were polymorphic，and the polymorphic rate was up to 78.1%. Using 12 specific bands in the 3 polymorphic ISSR primers（UBC889、 UBC812、 UBC825），the 9 velvet bean germplasm resources could be differentiated. The genetic similarity coefficients of velvet bean germplasm ranged from 0. 47 to 0. 97. Nine velvet bean varieties were divided into 3 groups by UPGMA cluster analysis with the similarity coefficient of 0. 73. The phylogenetic relationship of velvet beans are partly related to the geological sources of the germplasm.

Key words: velvet bean；germplasm resources, genetic diversity, ISSR, similarity coefficient, cluster analysis