The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

Journal of Jianghan University(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (2): 77-81.

• Orignal Article • Previous Articles Next Articles

The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

CHEN Jiang－yuan¹,LIU Jie²,CAI Jian－zhong¹,FU Yun－jie³,LIU Zhi－guo³

1. School of Health Science,Jianghan University,Wuhan,430016,Hubei,China;
2. College of Arts ＆ Science,Jianghan University,Wuhan,430056,Hubei,China;
3. School of Biotechnic and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China

Online:2012-04-20 Published:2013-11-07

Abstract

Abstract: Objective:To develop colloidal gold immunochromatographic(IC) method for rapidly detecting the concentration of Sodium Cholate. MethodsSodium Cholate－BSA immunogen was obtained via carbodiimide method. Then the immunoantigen was injected into New Zealand rabbits to prepare the anti－sodium cholate polyclona antibody according to routine immunity procedure. The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay (ELISA). ResultsThe sodium cholate－OVA antigen－colloidal gold compoundswere made for test strip preparation. It was proved that the specificity of the antibody was1 ∶ 80 000, and the detectability was 12 μg／mL by the method. ConclusionAccordingly, IC test strip method is effective and sensitive for rapidly detecting sodium cholate, which will probably have a broad clinical application prospect.

Key words: sodium cholate, polyclonal antibody, ELISA, colloidal gold, test strip

CLC Number:

R392.7

CHEN Jiang－yuan,LIU Jie,CAI Jian－zhong,FU Yun－jie,LIU Zhi－guo. The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(2): 77-81.

References

[1]翟兴红,张声生,李乾构.中西医结合治疗胆石症[J].世界华人消化杂志,2001,9(9)1038－1040.
［2]Johansen C, Chow W H, Jorgensen T, et al.Risk of colorectal cancer and other cancers in patients with gall stones [J].Gut, 1996, 39(3)439－443.
［3]吕煊.血清胆汁酸测定在肝病诊断中的意义[J].当代医学,2009,15(22)29－30.
［4]马开敏,袁鹏.中西医结合三步法治疗胆囊结石[J].中国中西医结合外科杂志,2000,6(2)78－80.
［5]倪文伟,顾猛,曹小秋,等.血清总胆汁酸测定在肝胆疾病中的临床意义[J].实用全科医学,2007,8(8)736－737.
［6]徐雷鸣,宗春华,张敏经,等.胆结石患者血清结合胆汁酸测定的临床研究[J].内科学杂志,2000,17(1)35－36.
［7]郭利军,王晶.三种免疫血清制备方法的比较[J].大连学院学报,2004,5(3) 8－10.
［8]周兰香,王宗元.磺胺对甲氧嘧啶全抗原的制备研究[J].江苏农业研究,2001,22(4) 55－58.
［9]Pattarawarapan M, Nangola S, Cressey T R, et al.Development of a one－step immunochromatographic strip test for the rapid detection of nevirapine (NVP), a commonly used antiretroviral drug for the treatment of HIV／AIDS[J].Talanta,2007,71(1)462－470.
［10]Simabuco F M,Carromeu C,Farinha－Arcieri L E,et al.Production of polyclonal antibodies against the human respiratory syncytial virus nucleoprotein and phosphoprotein expressed in Escherichiacoli[J].Protein Expression and Purification, 2007,53(1)209－215.
［11]张建群,刘翔,杨杨,等.ELISA法测定氨苄青霉素用多克隆抗体的制备与鉴定[J].中国食品学报,2006, 6(2)11－18.
［12]Kia S, Omidfar K, Kashanian S, et al.Development of rapid one－step assay for detecting digoxin toxicity [J].Toxicology Letters,2007,172 S82－S83.
［13]陶义训.免疫学和免疫学检测[M].北京人民卫生出版社,2001.
［14]李余动,张少恩,吴志刚,等.胶体金免疫层析法快速检测氯霉素残留[J].中国食品卫生杂志, 2005(5) 416－419.

The Study on the Preparation of Sodium Cholate Polyclonal Antibody and its Fast Determination Method

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments