Study on the Effects of Polyvinylpyrrolidone in Different Molecular-weight on the Efficiency of Polymerase Chain Reaction

Abstract

Abstract: The effect of different molecular－weight Polyvinylpyrrolidone (PVP) on PCR efficiency has been researched into by undertaking the 110 bp, single－stranded DNA amplification with quantitative real－time polymerase chain reaction (QPCR). The results indicates that 0.5％ PVP K－30, PVP 8000, PVP 58000 and PVP 630000 could effectively enhance the PCR efficiency, and the ability of different molecular－weight PVP to improve PCR efficiency is PVP 630000, PVP 58000, PVP K－30 and PVP 8000, respectively. However, PVP K－40 could significantly inhibit the generation of PCR product. In addition, the results of melting curve showed that 0.5％ PVP 630000 could dramatically reduce the Tm value of PCR product. It suggestes that PVP 630000 could interact with DNA template to reduce the stability of DNA conformation, promote the unwinding of DNA template in the denaturation stage of PCR, then enhance the PCR efficiency. The results reveal that the effects of different PVP on the PCR efficiency is closely related to the molecular－weight.

Key words: different molecular－weight PVP, PCR efficiency, DNA conformation

CLC Number:

LI Jia,LIN Yan,YAO Jing,JIANG Lin,LI Zheng,LIANG Yong. Study on the Effects of Polyvinylpyrrolidone in Different Molecular-weight on the Efficiency of Polymerase Chain Reaction[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(2): 69-73.

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