Abstract: Objective To meet the demand for large-scale extraction of peripheral blood DNA，we make improvements in the existing kit extraction method. Methods Eighty blood samples from healthy people in Wuhan were randomly divided into two groups with 40 samples in each group. Improved whole blood DNA extraction method and PBMC（peripheral blood mononuclear cell） DNA extraction method were used to extract DNA respectively， and their validity and quality were compared. Results By comparing and analyzing the experimental time，the quality and purity of the extracted DNA，and the PCR results， it was found that the improved whole blood DNA extraction method took significantly less time than the whole blood PBMC DNA extraction method， and the extracted DNA quality was significantly higher than the whole blood PBMC DNA extraction method（P < 0. 05），while the OD260/OD280 values of the genomic DNA extracted by the two methods were not statistically different（P > 0. 05），and single and bright bands can be seen in the PCR products of the two methods. Conclusion The improved whole blood DNA extraction method is a fast，economical，and efficient DNA extraction method，which can play a role in clinical transplantation matching and clinical genetic diversity detection.

Key words: improved whole blood DNA extraction method, PBMC DNA extraction method from whole blood, genomic DNA