Journal of Jianghan University (Natural Science Edition) ›› 2019, Vol. 47 ›› Issue (5): 454-458.doi: 10.16389/j.cnki.cn42-1737/n.2019.05.011
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ZHOU Xiaoyea,YUAN Junyongb,WANG Anb,JIANG Xiwangb,ZHEN Hongyan*a
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Abstract: Objective This experiment is conducted to select the appropriate concentration and time of serum and colchicine to synchronize breast cancer MCF- 7 cells at G0/G1 and G2/M phases, respectively. Methods MCF-7 was treated with different serum concentration to induce cell at G0/G1 stage synchronization,and treated with colchicine reagents to induce cell at G2/M synchronization. Cell cycle distribution was analyzed by flow cytometry for 12,24,36 h. Results After 24 hours treated,in 1% serum concentration group,the percentage of MCF-7 at G0/G1 accounted for(72. 96 ± 0. 84)%;in 1. 0 μg /mL colchicine reagent group,the percentage of MCF-7 at G2/M stage was(73. 67 ± 1. 77)%. 36 hours after treatment,in experiment groups of 1% serum and the group of colchicine, cell apoptosis was induced. Conclusion In experimental groups,1% serum treating and 1. 0 μg/mL colchicine regents treating for 24 h can basically completed cell cycle synchronization at G0/G1 and G2/M phases,respectively.
Key words: serum starvation, cell cycle synchronization, colchicine regents, apoptosis, G0/G1, G2/M
CLC Number:
R737.902
ZHOU Xiaoye,YUAN Junyongb,WANG An,JIANG Xiwang,ZHEN Hongyan. Application of Flow Cytometry on Cell Cycle Synchronization of Humun Breast Carcinoma MCF-7 Cell[J]. Journal of Jianghan University (Natural Science Edition), 2019, 47(5): 454-458.
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URL: https://qks.jhun.edu.cn/jhdx_zk/EN/10.16389/j.cnki.cn42-1737/n.2019.05.011
https://qks.jhun.edu.cn/jhdx_zk/EN/Y2019/V47/I5/454