Abstract: ObjectiveTo study the association between HBV－DNA level and HBV markers in hepatitis B patients. MethodsA total of 335 hepatitis B patients were recruited in this study. Serum HBV－DNA level was quantified by fluorescence quantitative polymerase chain reaction(FQ－PCR)and serum HBV markers were detected by enzyme－linked immunosorbent assay(ELISA) separately. The association between HBV－DNA level and HBV markers was primarily investigated by statistical analysis. ResultsThe HBV－DNA positivity and level were significantly higher in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients than those in other groups(P ＜ 0. 01); The HBV－DNA positivity and level were significantly lower in HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients than those in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients(P ＜ 0. 01), but the differences were no statistical significance between HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients and HBsAg(＋)、HBcAb(＋) patients(P ＞ 0. 05); HBV－DNA positivity was significantly higher in HbeAg(＋) patients than in HbeAg(－) patients(P ＜ 0. 01). ConclusionCombining quantitative detection of HBV－DNA with HBV markers, in order to provide a reliable basis for judging the hepatitis B infection, virus replication, infectious intensity and the effect of antiviral observations.

Key words: HBV－DNA, fluorescence quantitative polymerase chain reaction (FQ－PCR), HBV markers