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Table of Content

    20 April 2023, Volume 51 Issue 2
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    Preparation of CuO/In2O3 Nanocomposite and Its n-Butanol Gas Sensing Properties
    DU Rui，ZHAO Mengzhe，ZHOU Di，NIU Xiaojuan，TIAN Yu，ZHENG Guang，TU Yafang
    2023, 51(2):  5-14.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.001
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    The unique barrier effect of the p-n junction can not only significantly change the optical and electrical properties of semiconductor composites，but also affect the sensitivity of gas sensors. In this paper，CuO/In2O3 composite nanomaterials with different mass ratios were prepared by the two-step hydrothermal method. The morphology and structure of the materials were analyzed by X-ray diffraction，scanning electron microscopy，transmission electron microscopy，and X-ray photoelectron spectroscopy. It was observed that CuO was a spherical hierarchical structure，and In2O3 nanoparticles were attached to its surface.Based on the gas sensing performance test，it was found that when the mass ratio of CuO and In2O3 in CuO/In2O3 composite nanomaterials was 1∶2，its sensitivity to n-butanol with a volume concentration of 100 × 10-6 was 107 at 225 ℃ ，which was 57% higher than that of pure In2O3 nanoparticles. In addition，the sample also showed good selectivity and stability to n-butanol.
    Research Progress on Preparation，Modification，and Application of ZrO2 Aerogel Materials
    WANG Haiping，LIU Yun，HU Siqian
    2023, 51(2):  15-26.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.002
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    ZrO2 aerogel is a kind of nano-porous material with a 3D cross-linked network on the main body of ZrO2. Because of its acid-base duality，redox property，high specific surface area，high porosity，and good thermal and chemical stability，ZrO2 aerogel has a wide range of applications in thermal insulation，catalysis，adsorption，energy storage，and other fields. In this paper，the preparation process and drying method of ZrO2 wet gel were summarized. The representative studies and research progress of ZrO2-based aerogel modification were introduced from the aspects of doping modification and binary compounding. The research advances on ZrO2 aerogel materials in the fields of heat insulation， catalysis， and adsorption was reviewed. Finally， a prospect on the future research of ZrO2 aerogel was given.
    Responses of Seed Germination and Seeding Growth Physiology of 3 Pea Variants to Drought Stress
    YOU Qiang，ZHANG Hong，ZONG Shan，WANG Yuansong，WANG Chenghai，LI Weiling，SUN Binlian，QIAO Jialu
    2023, 51(2):  27-34.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.003
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    Objective As SARS-CoV-2 spreads around the world，an in-depth study of virus-host interaction is essential for exploring SARS-CoV-2 pathogenesis. MethodsThe SRAS-CoV-2 nonstructural protein Nsp1-Nsp16 was cloned by PCR and homologous recombination assay into a eukaryotic expression vector，and Nsp1-Nsp16 protein expression was detected by Western blot. Then，the influence of Nsp2 exerted on total ubiquitin levels was determined by Western blot，and the distribution of Nsp2 in the cells was found by immunofluorescence. Finally，co-immunoprecipitation was used to detect the interaction between Nsp2 and ubiquitin. ResultsWestern blot analysis revealed that Nsp1-Nsp16 could express smoothly in A549 cells. Meanwhile，it was shown that Nsp2 increased total ubiquitin expression in the cytoplasm. Co-immunoprecipitation experiments results declared that Nsp2 could interact with ubiquitin protein. ConclusionWe successfully construct the expression vector of nonstructural protein Nsp1-Nsp16，and find that Nsp2 can increase the total ubiquitin level of cells and be ubiquitinated. This study demonstrates that Nsp2 is involved in the interaction between SARS-CoV-2 and host via the ubiquitin pathway，which lays a foundation for further research on the mechanism of SARS-CoV-2 and host.
    Bibliometric Analysis of Treating COVID-19 with Combined Traditional Chinese and Western Medicine Based on CiteSpace
    YUAN Fahu，ZHANG Jiaqi，HAO Yun，ZHOU Xian
    2023, 51(2):  35-43.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.004
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    Objective This study focused on the systematic literature measurement and visual analysis of treating COVID-19 with combined Chinese and Western medicine to explore the development status，research hot spots and trends，and cutting-edge dynamics. It provides data，information support，and development references for treating COVID-19 with combined Chinese and Western medicine. Methods Three major databases of China National Knowledge Infrastructure （CNKI）， Wanfang Data， and China Science and Technology Journal Database were used as data sources to retrieve the relevant literature on the combined Chinese and Western medicine for the treatment of COVID-19 from March 2019 to March 2022. Statistical analysis was performed using bibliometric methods and CiteSpace software. Visual analysis was performed on publishing trends，research institution cooperation， author cooperation， keyword co-occurrence and clustering， and research hotspots. Results A total of 476 articles were included in this study，with the largest number published in 2020. Statistical analysis of published units showed that the cooperative relationship between institutions was discrete，indicating that the research on treating novel coronavirus with combined Chinese and western medicine was extensive. Hot spots in the research were mainly reflected in four aspects：coronavirus，diagnosis and treatment plan， combined Chinese and Western medicine，and epidemic prevention and control. Conclusion The research theme of treating COVID-19 with combined Chinese and Western medicine dynamically changes with the epidemic development，which is guided by clinical efficacy. It mainly focuses on the formulation of diagnosis and treatment plans. The cooperation between authors and institutions is insufficient，and communication needs to be further strengthened.
    Investigation on KAP and Satisfaction Related to COVID-19 Prevention and Control in Urban and Rural Residents in Zunyi City
    CHU Xiangyuan，ZHANG Rong，YUAN Ping，QI Guojia，SHI Shangpeng，SHI Xiuquan
    2023, 51(2):  44-50.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.005
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    Objective To investigate the knowledge，attitude，and practice（KAP）related to COVID-19 and the satisfaction with the prevention and control of COVID-19 in urban and Rural Residents in Zunyi City，and provide a reference for the health education and prevention and control of COVID-19. Methods Through online and offline questionnaires， the stratified multistage sampling method was used to investigate the KAP and satisfaction of urban and rural residents in Zunyi City for the prevention and control of the COVID-19 epidemic，and to analyze the differences and influencing factors of KAP among residents with different characteristics. Results A total of 603 valid questionnaires were collected. The total awareness rate of COVID-19 among residents was 79. 43%. There was a significant difference in the awareness of COVID-19 among different ages，educational levels，and occupations （P < 0. 05）. The proportion of residents with a positive attitude towards COVID-19 was 87. 23%，while there was no significant difference in the attitude of residents with different characteristics（P > 0. 05）. Moreover，93. 37% of them had good behavior in preventing the COVID-19 virus，while the differences among residents with different education and occupation were statistically significant（P < 0. 05）. The result of multivariate analysis showed that residents with low education levels had lower cognition of COVID-19 than those with high education levels OR = 0. 647（95%CI 0. 548，0. 764）. The residents with high cognition took better protective behavior than those with low cognition OR = 0. 531（95%CI 0. 328，0. 860）. Conclusion Urban and rural residents have a high degree of satisfaction，a good KAP，and a high level of enthusiasm for COVID-19 prevention and control. However，it is necessary to strengthen the publicity and education of COVID-19 in vulnerable groups （such as low education levels and older/younger） to better promote the precise prevention and control of COVID-19.
    Resistant Detection of Echinochloa Crus-galli from Rice Fields to Penoxsulam in Anhui Province
    GU Chengwen，ZHAO Yuanyuan，DU Fengyang，DAI Weizhen，XU Jincheng，BI Yaling
    2023, 51(2):  51-60.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.006
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    In order to detect the resistance level of Echinochloa crus-galli（E. crus-galli） from rice fields to penoxsulam in Anhui province and its distribution，whole-plant bioassay experiments were conducted to evaluate the resistance level of 40 populations of E. crusgalli from rice fields in different areas of Anhui province. Additionally，the resistant biotype with the highest resistance level was tested for sensitivity to 15 herbicides. The results showed that E. crus-galli in Anhui rice fields had varying degrees of resistance to penoxsulam. Among the tested samples，20 resistant populations accounted for 50% of the total number，including 13 medium and high-resistant populations accounted for 31. 5% of the total number. The resistance levels of E. crus-galli cwere much higher in the plain area along the Yangtze River and the Jianghuai hilly region，followed by a mountainous area of southern Anhui. In contrast，the resistance levels in plain regions in Huaibei Plain and Dabie Mountains were much lower. The resistant population R was resistant to metazosulfuron， bispyribac-sodium，quinclorac，cyhalofop-butyl，tripyrasulfone，sensitivity to pyraclonil， oxadiazon，thiobencarb，anilofos，and metamifop was lower. At the same time，it was sensitive to pretilachlor，butachlor，pendimethalin，butralin，and propanil.
    Inhibitory Activity of the Combination of Biocontrol Metabolites and Fungicides Against Peanut Brown Spot Pathogen
    YANG Shengyu，YANG Fei，DUAN Haiming ，CHEN Su，ZHOU Hui，ZHANG Jian
    2023, 51(2):  61-67.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.007
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    Peanut brown spot is a leaf disease caused by Cercospora arachidicola （C.arachidicola）. To explore the inhibitory activity of the mixture of biocontrol metabolites and chemical fungicides，the antagonistic effect of biocontrol bacterium SJ1606 on C. arachidicola was tested by confrontation culture method，and then the inhibitory activity of antimicrobial lipopeptides，six chemical fungicides and the mixture of lipopeptides and chemical fungicides were determined by mycelial growth rate method. The results showed that the antagonistic effect of SJ1606 on C. arachidicola was significant，and the inhibition rate was 71. 8%. The EC50 of crude antimicrobial lipopeptide extract was 1 149. 89 mg/L. Carbendazim was the best among the six chemical fungicides，with EC50 of 1. 53 mg/L. The EC50 of prochloraz， tebuconazole，propiconazole and pyraclostrobin were 1. 74，2. 15，4. 50 and 5. 40 mg/L respectively. The antifungal activity of difenoc onazole was the weakest，with EC50 of 13. 14 mg/L. The mixture of lipopeptides and chemical fungicides showed a synergistic effect in a certain proportion. The mixture of lipopeptides and propiconazole showed a synergistic effect on the inhibitory activity of C. arachidicola，and the toxicity ratio was up to 1. 39 when the volume ratio was 1∶9. The other five chemical fungicides mixed with lipopeptide had both synergistic and antagonistic effects. When the ratio of carbendazim to lipopeptide was 2∶8，the highest toxicity ratio was 1. 35. In summary，the metabolites of biocontrol bacteria and the tested chemical fungicides showed good inhibitory activity against C. arachidicola， and the mixture of propiconazole and lipopeptide had better inhibitory activity against C. arachidicola. This study will provide technical support for the utilization of biocontrol bacteria SJ1606 and the scientific use of fungicides.
    Research on Digital Twin Modeling Method of Manufacturing Line Under Industry 4.0 Background
    XUAN Liang，ZHANG Chunfei，LIU Xiaohui，YANG Peijie
    2023, 51(2):  68-77.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.008
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    The rapid development of the cyberphysical system （CPS） promotes the rapid transformation and upgrading of the traditional manufacturing industry. To further improve the CPS establishment of the intelligent manufacturing production line，this paper compared and analyzed the research on the definition of the digital twin by relevant organizations and institutions at home and abroad. The digital twin of an intelligent manufacturing production line was built by TIA Portal software， whose unmanned CPS was composed of the modulations of production，manufacturing，processing，transport，and warehouse. The experiments were carried out based on the finished product warehouse，which verified the feasibility of the CPS and provided data and a practical basis for the subsequent iterative upgrading of the intelligent manufacturing production line.
    Research on Adaptive Trajectory Tracking Control Strategy for Unmanned Surface Vehicle
    LIU Zhiqiang，YE Xi，ZHANG Zhiwei
    2023, 51(2):  78-89.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.009
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    An adaptive trajectory tracking control strategy was proposed to address the problems of speed loss caused by rudder maneuvering and tracking error becoming larger due to steering during the unmanned surface vehicle trajectory tracking. In the guidance loop， the admittance circle radius of the track points was adjusted according to the angle between the track segments based on the adaptive admittance circle strategy，and the adaptive line of sight（LOS）guidance law was designed to improve the tracking accuracy of the unmanned surface vehicle when steering. In the control loop，the decoupling control strategy of heading and speed based on model predictive control（MPC）was proposed，which first considered the coupling term of the heading model and speed model as a time-varying disturbance and proposed a new time delay estimation method，then predicted it by the heading controller， and finally compensated for the time-varying disturbance in the speed controller to reduce the speed loss. After that，the feasibility of the algorithm was verified by the MATLAB simulation platform. The experimental results showed that the proposed adaptive trajectory tracking control strategy had higher tracking accuracy，faster convergence speed，better anti-interference capability，and less energy consumption.
    Research and Application of Horizontal Well Infilling Technology in Lowefficiency Well Area of Shizhuang South Coalbed Methane Field
    DUAN Baojiang，SUN Yuting，LIU Chengzhen，WANG Wensheng，LI Jianrong
    2023, 51(2):  90-96.  doi:10.16389/j.cnki.cn42-1737/n.2023.02.010
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    Most coalbed gas fields in China have poor physical properties，permeability is generally less than 1. 0 mD，and the well pattern and well spacing is too large，which leads to difficulties in regional coordinated depressurization and production of gas well is low. Infill adjustment of the horizontal well is one of the effective key technologies to solve the production increase of coalbed gas wells. Taking Shizhuang south block as the research object，this paper evaluated the rationality of well pattern and well spacing in this area using well test interference and numerical simulation，optimized the potential area of horizontal well infilling according to reservoir geological parameters，designed the adjustment scheme of horizontal well infilling，and applied it. The results showed that after the infill adjustment of horizontal wells，the distance between coalbed methane（CBM） wells was shortened， effective communication between regional gas wells was formed，and regional depressurization was formed. The stable daily gas production of horizontal wells was 3 600 m3 ，which was 2. 5 times of surrounding gas wells. The average production increase of single wells in surrounding gas wells was 300 m3/d，and remarkable results were achieved.