Abstract: ObjectiveTo evaluate the application value of liquid－based cytology and HPV 16／18 detection by in situ hybridization in cervical lesions. Methods112 cases were examined with liquid－based thin－layer cytology and stained with HE. According to the classification standard of TBS, the cervical lesions were divided into negative for intraepithelial or malignancy 单位 NILM); atypical squamous cell of undetermined significance单位 ASC－US); Low－grade squamous intraepithelial lesion 单位 LSIL); High－grade squamous intraepithelial lesion 单位 HSIL) and squamous cell carcinoma 单位 SCC). And HPV 16／18 of 112 cases were detected by in situ hybridization. Results① In the 112 cases, 16 cases 单位 14.3％) were NILM; 26 cases 单位 23.2％) were inflammatory; 44 cases 单位 39.3％) were ASC－US; 25 cases 单位 22.3％) were LSIL; 1 case 单位 0.89％) was HSIL. ② With the lesion aggravating, the positive rate of HPV 16／18 increased gradually. And there was a significant difference 单位 P＜0.05) between NILM, inflammation and LSIL, HSIL. ③ In cervical cytology, HPV 16／18 positive expression was located in cytoplasm in 5 cervical biopsy specimens. And it was also located in nucleus detected by in situ hybridization in the same specimens. ConclusionsLiquid－based cytology combining with HPV 16／18 detection by in situ hybridization is helpful for testing cervical lesion; cytoplasm HPV 16／18 positive staining may be used as a positive assessment marker in cervical cytology.

Key words: liquid－based cytology, HPV16／18, cervical cytology