Combined Effect Study of C60／Cu2＋ Affecting Plasmid DNA Conformation

Abstract

Abstract: The toxic effects of carbon nanoparticles have been increasingly gaining attention. In this research, the combined effect of C60／Cu2＋ on DNA conformation was comparatively studied by altering various reaction conditions, to assess the biological toxicity of carbon nanoparticles. The results showed that without photoexcitation, C60／Cu2＋ could significantly induce the conversion of supercoiled DNA into nicked DNA, and this DNA cleavage effect was enhanced as the concentration of Cu2＋ and C60 increased. Moreover, the cleaved DNA could be reconnected to circular DNA by T4 DNA ligase, which indicated that the phosphodiester bond was the splicing site of C60／Cu2＋. In addition, various ROS savengers could not reduce the DNA cleavage effect of C60／Cu2＋, which was relate to the feature that Cu2＋ played a pivotal role in DNA cleavage. Taken together, these results demonstrated that C60／Cu2＋ could significantly induce the DNA cleavage without photoexcitation, which exhibited obvious combined effect. Further consideration should be given in assessment of their potential genotoxicity.

Key words: Cu2＋, C60, T4 DNA ligase, ROS

CLC Number:

Q555.4

LI Jia,LIN Yan,LI Zhun－jie,LI Zheng,LIANG Yong. Combined Effect Study of C60／Cu2＋ Affecting Plasmid DNA Conformation[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(5): 80-84.

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