Abstract: The effects of different media and culture methods on aseptic germination of Bletilla striata seeds，the effects of different size seedling bags on the survival of Bletilla striata aseptic seedlings，and the influence of shade intensity on the growth of Bletilla striata container seedlings were studied. The results showed that there was no significant difference in germination rate of Bletilla striata seeds in different media and culture methods，but the corm in solid BJ4 medium was larger than that other treatments；the average survival rate and growth of the aseptic seedlings treated with larger size（16 cm × 20 cm）seedling bags were better. In terms of shading strength，the four-needle shading net was more conducive to the survival and growth of Bletilla striata. The survival rate of Bletilla striata was 60% and the average seedling height was 5. 65 cm after transplanting for 2 months. However，the unshaded state was not suitable for the growth of Bletilla striata container seedlings.

Key words: Bletilla striata, aseptic seedlings, transplanting, shading intensity