Abstract: In this paper，the bacteriostatic effects of Artemisia selengensis tea extract with different solvent on six strains（Escherichia coli， Bacillus subtilis， Staphylococcus aureus， Pseudomonas aeruginosa，Proteus vulgaris，Bacillus aerogenes）were studied with orthogonal design method. The results showed that the extract with 90% ethanol solution at 95 ℃ for 75 min had the biggest bacteriostasis rings on Escherichia coli，Bacillus subtilis，Proteus vulgaris and Bacillus aerogenes，respectively，and under this condition，the bacteriostasis rings of Staphylococcus aureus and Pseudomonas aeruginosa had higher value，therefore the bacteriostatic effects were better under this condition. According to the water extraction test，the best antibacterial effect was obtained under the condition of distilled water being soaked for 2 hours at 85 ℃. The analysis showed that the inhibitory effects of the extract of Artemisia selengensis tea on different bacteria were not significant，which indicated that it had broad spectrum bacteriostatic effect. Among the six kinds of bacteria，the extract of Artemisia selengensis tea had the least inhibitory concentration to Escherichia coli，this indicated that it had better inhibitory effect on Escherichia coli.

Key words: Artemisia selengensis tea, ethanol extraction method, water extraction method, bacteriostatic effect