Abstract: Objective To figure out the difference in blood-test results of venous blood and peripheral blood and provide the needed experimental data and theoretical basis to develop the standard operation procedure for routine blood testing. Methods This research took blood samples of 128 healthy college students participating in the physical examination，summarized and analyzed their biochemical results including white blood cell（WBC），red blood cell（RBC），hemoglobin（HGB），blood cell hematocrit（HCT），mean red cell volume（MCV），mean corpuscular hemoglobin content（MCH），mean erythrocyte corpuscular hemoglobin concentration（MCHC）and platelet（PLT）. Results WBC，RBC，HGB，HCT and PLT results of venous blood and peripheral blood were statistically significant（ P < 0. 05），and there were no significant difference in MCV，MCH and MCHC results（ P > 0. 05）. Conclusion There are noticeable differences in the results of blood routine examination of peripheral blood and venous blood. The test results of venous blood is less susceptible to the effects of temperature and peripheral circulation change，hence it can more accurately reflect the real blood situation of the entire body， they are more representative.

Key words: venous blood, peripheral blood, routine blood-test, results comparison and analysis