Journal of Jianghan University(Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (3): 248-252.

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Preliminary Establishment of Rapid Propagation System of Cymbidium Hybridum

HU Yanmei1， XIA Jinbo2， FANG Zhongming*3， GUO Yungui3， YANG Yonghui3   

  1. 1. Periodicals Press，Jianghan University， Wuhan 430056， Hubei， China；2. School of Life Science and Technology，Jinan University， Guanghzou 510632，Guangdong，China；3. Life Science and Technology College，Wuhan Bioengineering Institute， Wuhan 430415，Hubei，China
  • Online:2015-06-28 Published:2015-07-02

Abstract: The formation effects of protocorm of cymbidium induced by four kinds of explants are discussed，as well as the influence of hormone proportion to proliferation and differentiation of protocorm induction. The experiment results showed the best explants for protocorm induction were stem tips，the inducing rate reached 76. 0% after 60 days culture. The three other explants could not effectively induce the protocorm. With 0. 5 mg/L 6-BA +0. 1 mg/L NAA treatment，the formation rate of protocorm induced by stem tip reached 87. 8%，and the proliferation rate of protocorm induced by stem tip with each hormone treatment all reached 100%，the proliferation and differentiation of protocorm were the best with the treatment of 1. 5 mg/L KT + 0. 5 mg/L IBA，the proliferation coefficient and the differentiation coefficient are 5. 19 and 2. 80， respectively.

Key words: cymbidium, rapid propagation, stem tip, protocorm, proliferation and differentiation

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