Abstract: Objective To know the sub-health status and variation tendency of Jianghan University students. Methods With the same human energy detector（sub-health detector）which is made in German to detect bioelectricity feedback repeatedly of 168 students from April to May in 2012 and the same period in 2013. Made a tracing research for sub-health situation of the students and conducted a questionnaire investigation for their daily life. Results In the tested students，60% of the male students and 81.5% of the female students were under sub-health condition. Younger the tested male students were，higher their sub-health situation percentages were；on the contrary，older the tested female students were，higher their sub-health situation percentages were. From variation of their sub-health in a year， only 4.8% of the students turned to be better， however， 42. 9% of the students changed from healthy situation into sub-health condition. What′ s more，for their daily life，those physical situations have improved and remained health conditions were better than those physical situation turned to be bad and remained sub-health condition generally. There were some causes for their sub-health situation as follows：firstly，their sleep quality was not very good；secondly，they were under strong pressure because of employment and other reasons；thirdly，lacking of systematic health education leaded to unscientific and unhealthy lifestyle. Conclusions It is alarming for the physical situation of college students， most of the college students were under sub-health situation or transforming into the sub-health situation. The universities should develop healthy education for students actively and take measures to protect physical and mental health for students.

Key words: students, sub-health, investigation