Journal of Jianghan University (Natural Science Edition) ›› 2024, Vol. 52 ›› Issue (3): 25-31.doi: 10.16389/j.cnki.cn42-1737/n.2024.03.003

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Effect of Salt Stress on Growth,Physiological and Biochemical Properties of Sprouting Cuttings of Artemisia selengensis

XUE Tianyuan,ZHANG Qianqian,WANG Yuhang,HE Sixiao,DONG Yuanhuo,DAI Xigang,ZENG Changli*   

  1. School of Life Sciences;Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,Jianghan University,Wuhan 430056,Hubei,China
  • Published:2024-06-17
  • Contact: ZENG Changli

Abstract: The wild and cultivated species of Artemisia selengensis collected from the Xiangkou base in Hannan,Wuhan,China,were subjected to three concentration gradients of 0,20,and 40 mmol/L NaCl solution,with the Hoagland nutrient solution without salt stress as the control. The half-lethal concentration of Artemisia selengensis cuttings was screened out, and the effect of salt stress on growth and physiological indicators of Artemisia selengensis cuttings was studied. The results showed that salt stress significantly reduced the survival rate of cultivated and wild species Artemisia selengensis cuttings compared to the control,with a half-lethal concentration of 53. 40 mmol/L for wild species Artemisia selengensis cuttings and 50. 99 mmol/L for cultivated species Artemisia selengensis cuttings. Under NaCl stress,the plant height,root length,and lateral root number of wild species Artemisia selengensis cuttings were not significantly inhibited and showed a trend of first increasing and then decreasing;the plant height,leaf number,root length,and lateral root number of cultivated species cuttings were significantly inhibited;the CAT,POD,APX,and SOD activities of the wild species Artemisia selengensis cuttings significantly increased under the influence of salt stress,and the MDA and proline contents showed a trend of first decreasing and then increasing;the activities of CAT,POD,APX,and SOD were significantly increased,and the contents of MDA and proline were significantly increased in the cuttings of cultivated Artemisia selengensis with the increase in salt concentration. In contrast,the activities of antioxidant enzymes(CAT,POD,and SOD)in the cuttings of wild species Artemisia selengensis were lower than those of cultivated species Artemisia selengensis. It can be assumed that the cultivated species of Artemisia selengensis are more vulnerable to salt stress and that the wild species of Artemisia selengensis cuttings are more salt tolerant.

Key words: Artemisia selengensis, salt stress, sprouting cuttings, physiological index, NaCl solution

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