Abstract: Objective To measure and compare the tissue oxygenation of gastrocnemius during ischemic and reperfusion（I & R） to lower limbs through two different ways，and to establish a method for remote ischemic preconditioning（RIPC）through intermittent seiza and observe its effect on capillary fragility of forearm skin. Methods 1）12 college students participated in the measurement of tissue oxygenation in gastrocnemius during I & R either through intermittent seiza or blood pressure cuff inflated at 200 mmHg around thigh，taken 5 min reperfusion following 5 min ischemia as a cycle，three such cycles was one RIPC. 2）The capillary fragility was checked in the forearm of 61 students whom were randomly into experimental group（34）and control group（27）at pretreatment， two weeks（partial cases）and four weeks after the daily intermittent seiza RIPC. The BP and platelet （platelet 14 cases in each group）were checked while checking the capillary fragility. Results Tissue oxygenation in gastrocnemius changed regularly during I & R in both methods，but seiza could make it decrease more rapidly and thoroughly than that of blood pressure cuff inflation. The skin capillary fragility reduced significantly compared with that of the control group after 4 weeks daily intermittent seiza RIPC. No significant changes were observed for BP and platelet count. Conclusion Intermittent seiza is an effective and easy way for RIPC；it can improve the quality of skin capillary.

Key words: intermittent seiza, remote ischemic preconditioning（RIPC）, gastrocnemius, tissue oxygenation, capillary fragility