Abstract: To clarify the distribution of quality genes in 151 wheat varieties （lines） of Jiangsu Ruihua Agricultural Science and Technology Co. ，Ltd. ，molecular markers were used to detect high molecular weight glutenin subunit（HMW-GS），PPO activity，and 1B/1R ectopic lines. The results showed that the gene frequencies of HMW-GS Ax2， Dx5，By8，and Bx7 were 1. 32%，17. 22%，70. 20% and 89. 40%，respectively. The gene frequencies of Ax2 + Dx5，Ax2 + By8，and Ax2 + Bx7 were all 0. 66%，and the gene frequencies of Dx5 + By8，Dx5 + Bx7，and By8 + Bx7 were 7. 28%，14. 57% and 62. 25%，respectively. The frequency of polyphenol oxidase（PPO）activity-related genes Ppo-A1b and Ppo-D1a was 45. 70% and 25. 83%，double low PPO activity allele PpoA1b + Ppo-D1a was an excellent allele，with a frequency of 8. 60%. The distribution frequency of 1B/1R was 86. 75%. Only 2 materials were carrying low PPO activity allelic variation combinations and non-1B/1R ectopic lines at the same time，with a frequency of 1. 32%. Only one material was carrying an allelic variation combination of glutenin subunit Bx7 and low PPO activity at the same time，which was a non-1B/1R ectopic line，and it could be used as wheat breeding material.

Key words: wheat, high molecular weight glutenin, polyphenol oxidase, 1B/1R ectopic line, molecular marker