关键词: 三苯胺-噻吩, 吲哚, CN^?检测, 荧光探针, 藻细胞

Abstract: Cyanide（CN^?）is one of the most well-known toxic anions，some edible algae can release cyanide or absorb cyanide from their living environments，which may cause harm to human health. Therefore，we designed and synthesized a novel fluorescent probe TI for CN^? detection in living algae cells. Probe TI was constructed by triphenylamine- thiophene moiety and indolium moiety and exhibited remarkable blue fluorescence at 473 nm upon treatment with cyanide due to the nucleophilic attack of CN^? on the C=N bond. Moreover，probe TI demonstrated excellent selectivity and sensitivity （LOD 95. 7 nmol/L），and a fast responsive time for cyanide detection in an aqueous solution. Most importantly，probe TI could be applied for real- time detection of CN^? in living algae cells and edible algae.

Key words: triphenylamine-thiophene, indolium, CN^? detection, fluorescence probe, algae cells