关键词: 菜豆, 组织培养, 子叶节, 再生苗, 炼苗移栽

Abstract: The effects of different concentrations of auxins IBA（indole butyric acid）， NAA（naphthalene acetic acid）and cytokines 6-BA（6-benzyl adenine），KT（kinetin）on the induction of adventitious buds and roots were studied from the cotyledonary node of "Bean white rice" common bean，and the common bean′s tissue culture seedlings were cultivated and transplanted. The results showed that the optimum medium for buds induction was MS + 1. 0 mg/L IBA + 0. 5 mg/L 6-BA + 0. 1 mg/L KT，the cofficient of the bud differentiation was 2. 89. The optimum rooting medium was MS + 0. 1 mg/L IBA，the rooting rate was 100%. The common bean′s tissue culture seedlings can survive after transplanting.

Key words: common bean, tissue culture, cotyledonary node, regenerated seedlings, cultivation and transplantation