江汉大学学报（自然科学版） ›› 2024, Vol. 52 ›› Issue (3): 25-31.doi: 10.16389/j.cnki.cn42-1737/n.2024.03.003

• 经济作物研究 • 上一篇    

盐胁迫对藜蒿扦插枝萌蘖生长及其生理生化特性的影响

薛天源，张千千，王宇航，何思晓，董元火，戴希刚，曾长立*   

  1. 江汉大学 生命科学学院；湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心，湖北 武汉 430056
  • 发布日期:2024-06-17
  • 通讯作者: 曾长立
  • 作者简介:薛天源（1999— ），男，硕士生，研究方向：特色植物资源开发与利用。
  • 基金资助:
    湖北省重点研发计划项目（2022BBA0064）；武汉市产学研项目（CXY202203）；江汉大学一流学科建设重大专项资助计划项目（2023XKZ026）

Effect of Salt Stress on Growth，Physiological and Biochemical Properties of Sprouting Cuttings of Artemisia selengensis

XUE Tianyuan，ZHANG Qianqian，WANG Yuhang，HE Sixiao，DONG Yuanhuo，DAI Xigang，ZENG Changli*   

  1. School of Life Sciences；Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin，Jianghan University，Wuhan 430056，Hubei，China
  • Published:2024-06-17
  • Contact: ZENG Changli

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摘要： 以武汉市汉南湘口基地采集的野生种藜蒿和栽培种藜蒿为试验材料，设置 NaCl 溶液为 0、20、40 mmol/L 3 个浓度梯度，以无盐胁迫处理的 Hoagland 营养液作为对照，筛选出藜蒿扦插 枝半致死浓度，研究盐胁迫对藜蒿扦插枝萌蘖生长指标和生理指标的影响。结果表明：与对照相 比，盐胁迫显著降低栽培种和野生种藜蒿扦插枝的存活率，野生种藜蒿扦插枝的盐胁迫半致死浓 度为 53. 40 mmol/L，栽培种藜蒿扦插枝的盐胁迫半致死浓度为 50. 99 mmol/L。在盐胁迫处理 下，野生种藜蒿扦插枝的株高、根长和侧根数受抑制不显著，并呈现出先升后降的趋势，栽培种扦 插枝株高、叶片数、根长和侧根数被显著抑制；野生种藜蒿扦插枝在盐胁迫影响下，CAT、POD、 APX、SOD 活性均显著提高，MDA 与脯氨酸含量呈现先降后升的趋势；而栽培种藜蒿扦插枝随 着盐浓度的上升，CAT、POD、APX、SOD 的活性显著增强，MDA 和脯氨酸含量也显著提高；野 生种藜蒿扦插枝抗氧化酶（CAT、POD、SOD）活性均低于栽培种。由此可以推测栽培种藜蒿扦 插枝应对盐胁迫伤害更大，野生种藜蒿扦插枝耐盐性更强。

关键词: 藜蒿, 盐胁迫, 扦插枝, 生理指标, NaCl溶液

Abstract: The wild and cultivated species of Artemisia selengensis collected from the Xiangkou base in Hannan，Wuhan，China，were subjected to three concentration gradients of 0，20，and 40 mmol/L NaCl solution，with the Hoagland nutrient solution without salt stress as the control. The half-lethal concentration of Artemisia selengensis cuttings was screened out， and the effect of salt stress on growth and physiological indicators of Artemisia selengensis cuttings was studied. The results showed that salt stress significantly reduced the survival rate of cultivated and wild species Artemisia selengensis cuttings compared to the control，with a half-lethal concentration of 53. 40 mmol/L for wild species Artemisia selengensis cuttings and 50. 99 mmol/L for cultivated species Artemisia selengensis cuttings. Under NaCl stress，the plant height，root length，and lateral root number of wild species Artemisia selengensis cuttings were not significantly inhibited and showed a trend of first increasing and then decreasing；the plant height，leaf number，root length，and lateral root number of cultivated species cuttings were significantly inhibited；the CAT，POD，APX，and SOD activities of the wild species Artemisia selengensis cuttings significantly increased under the influence of salt stress，and the MDA and proline contents showed a trend of first decreasing and then increasing；the activities of CAT，POD，APX，and SOD were significantly increased，and the contents of MDA and proline were significantly increased in the cuttings of cultivated Artemisia selengensis with the increase in salt concentration. In contrast，the activities of antioxidant enzymes（CAT，POD，and SOD）in the cuttings of wild species Artemisia selengensis were lower than those of cultivated species Artemisia selengensis. It can be assumed that the cultivated species of Artemisia selengensis are more vulnerable to salt stress and that the wild species of Artemisia selengensis cuttings are more salt tolerant.

Key words: Artemisia selengensis, salt stress, sprouting cuttings, physiological index, NaCl solution

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