江汉大学学报(自然科学版) ›› 2015, Vol. 43 ›› Issue (6): 538-543.doi: 10.16389/j.cnki.cn42-1737/n.2015.06.011

• 化学分析与材料科学 • 上一篇    下一篇

不同方法提取荸荠皮中总黄酮含量的比较

许国权,胡梦蝶   

  1. 江汉大学 生命科学学院,湖北 武汉 430056
  • 出版日期:2015-12-28 发布日期:2016-01-12
  • 作者简介:许国权(1974—),男,高级实验师,研究方向:天然物质提取。
  • 基金资助:
    湖北省教育厅科学技术研究项目(B2014073)

Comparison of Different Methods on Extracting Flavones in Water Chestnut Peel

XU Guoquan,HU Mengdie   

  1. School of Life Sciences,Jianghan University,Wuhan 430056,Hubei,China
  • Online:2015-12-28 Published:2016-01-12

摘要: 研究了不同方法提取荸荠皮中总黄酮类物质,在不同条件下对荸荠皮中的总黄酮物质进行提取,用正交优选法选出适宜的条件。结果表明:采用超声波辅助提取方法得到的总黄酮提取物最多,该方法的适宜提取工艺为:提取时间为50min,乙醇浓度为50%,提取温度为60℃,料液比为1∶30,该条件下,荸荠皮中总黄酮的得率为2.483%;其次是微波辅助法提取,该方法的适宜提取工艺为:功率为420 W,料液比为1∶30,乙醇浓度为50%,提取时间为5min,该条件下,荸荠皮中总黄酮的得率为2.455%;加热提取的最少,适宜提取工艺为:提取温度为60℃,乙醇浓度60%,料液比为1∶30,提取时间为4h,该条件下,荸荠皮中总黄酮的得率为1.618%。比较3种提取方法各有优劣,在实际工作中可以根据情况选择不同的提取方法。

关键词: 荸荠皮, 总黄酮, 加热提取, 微波提取, 超声波提取

Abstract: To research the different methods on extracting flavonoid from water chestnut peel,the research extracted flavonoid from water chestnut peel under different conditions,and choose the suitable conditions with orthogonal test. The results showed with ultrasonic heating method ,it extracted the most flavones,the suitable conditions in this method were heating time 50 min,50% ethanol,60 ℃,solid-liquid ratio of 1∶30,under the conditions,the yield of flavones was 2. 438% ;the secondary was microwave heating method,the suitable conditions were 420 W,solid- liquid ratio of 1∶30,50% ethanol,heating time 5 min,under the conditions,the yield of flavones was 2. 455%;with the bathing heating method,it extracted the least flavones,the suitable conditions were 60 ℃,60% ethanol,solidliquid ratio of 1∶30,heating time 4 h,under the conditions,the yield was 1. 618%. The three methods each has its advantage and disadvantage,can be used in different situation.

Key words: water chestnut peel, flavones, water bath heating, microwave heating, ultrasonic heating

中图分类号: