江汉大学学报(自然科学版) ›› 2015, Vol. 43 ›› Issue (5): 454-459.doi: 10.16389/j.cnki.cn42-1737/n.2015.05.015

• 生命科学 • 上一篇    下一篇

一株野生酿酒酵母的分离鉴定及发酵分析

阎贺静,时月,刘畅,李润丰   

  1. 河北科技师范学院 食品科技学院,河北 昌黎 066600
  • 出版日期:2015-10-28 发布日期:2015-11-02
  • 作者简介:阎贺静(1978—),女,副教授,博士,研究方向:发酵工程。
  • 基金资助:
    河北省高等学校科学技术研究项目(QN2014143);河北科技师范学院博士研究启动基金资助项目

Isolation,Identification and Fermentation Analysis of a Wild Saccharomyces Cerevisiae

YAN Hejing,SHI Yue,LIU Chang,LI Runfeng   

  1. College of Food Science and Technology,Hebei Normal University of Science and Technology,Changli 066600,Hebei,China
  • Online:2015-10-28 Published:2015-11-02

摘要: 本试验从玫瑰香葡萄酒自然酿造过程中筛选分离出一株野生酵母菌,经菌落形态观察及分子生物学鉴定为酿酒酵母,命名为Yeast1。对Yeast1进行乙醇耐受性分析,发现Yeast1能够耐受16%(v/v)的酒精度。Yeast1发酵葡萄汁结束后,残糖量、酒精度、挥发酸、总酯和总酸含量分别为3. 627 011 g/L、8. 5%、0. 210 84 g/L、1. 264 4 g/L和4. 160 3 g/L,分析表明Yeast1发酵力强、速度快、酒精产量较高,残糖量符合酿造干型酒的要求,满足正常的发酵需要。此外,从Yeast1葡萄汁发酵液中共检测到50 种香气成分,其中醇类18种、酯类13种、醛类7种、其他酸烯酮醚等物质12种。研究结果表明,昌黎产地玫瑰香葡萄自然酿造中存在较好性能的天然酵母,若将其合理利用可能会酿造出更优质、更具特色的葡萄酒。

关键词: 玫瑰香葡萄酒, 野生酵母, 分离, 鉴定, 发酵分析

Abstract: In this experiment,one Saccharomyces cerevisiae was screened and identified from Muscat wine natural brewing broth with morphological method and molecular technology,which was named as Yeast1. Firstly,Yeast1 could tolerate 16%( v/v )ethanol. Moreover,after the fermentation of grape juice by Yeast1,the content of residual sugar,of ethanol,of volatile acid,of total ester and of total acid were 3. 627 011g/L、8. 5% 、0. 210 84g/L、1. 264 4g/L and 4. 160 3g/L,respectively. These results indicated that Yeast1 has better fermentation ability,and can initiate the fermentation immediately and produce a higher content of ethanol. At the same time,the residual sugar in Yeast1 fermentation broth fit the standard for dry wine production. Furthermore,there are 50 kinds of aroma compounds were detected by GC-MS in Yeast1 fermentation broth,of which,there are 18 kinds of alcohol compounds,13 kinds of ester compounds,7 kinds of aldehyde compounds and other 12 kinds of compounds like acids,ketene and ether,and so on. These results indicated that there are yeasts with good fermentation properties in muscat wine natural process in Changli district,which maybe produce better wines in quality and characteristic if they were used appropriately.

Key words: muscat grapes wine, natural yeast, isolation, identification, fermentation analysis

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