酒精对大鼠外侧穿通纤维-CA3区LTP诱导的影响

江汉大学学报（自然科学版） ›› 2011, Vol. 39 ›› Issue (2): 66-69.

酒精对大鼠外侧穿通纤维-CA3区LTP诱导的影响

杨荣,杨友华,陈晓青,黄丹

江汉大学医学院基础部生理教研室, 湖北武汉 430056

收稿日期:2011-01-12 出版日期:2011-03-20 发布日期:2013-12-30
作者简介:杨荣(1975—),女,湖北仙桃人,讲师,博士,主要从事老年性痴呆发病机制的研究.
基金资助:
湖北省自然科学基金资助(2006AB198)

Effects of Alcohol on Induction of Long-term Potentiation in Lateral Perforant Path-CA3 Area of Rats

YANG Rong, YANG You-hua, CHEN Xiao-qing, HUANG Dan

Department of Physiology, School of Medicine, Jianghan University, Wuhan 430056, Hubei, China

Received:2011-01-12 Online:2011-03-20 Published:2013-12-30

摘要/Abstract

摘要： 目的研究酒精对大鼠外侧穿通纤维(lateral perforant path, LPP)-CA3区(LPP-CA3)长时程增强(long-term potentiation, LTP)诱导阶段的影响及可能的机制.方法用细胞外电生理记录方法, 通过向海马CA3区分别微量注射酒精 (Alcohol)、阿片受体拮抗剂纳洛酮(naloxone)和GABA_A受体拮抗剂荷包牡丹碱(bicuculline, BIC),以海马CA3区群体兴奋性突触后电位(field excitory postsynaptic potentials, fEPSP)斜率改变为指标, 观察高频刺激引起的LTP诱导的变化.结果① 酒精剂量依赖性地抑制 LTP的诱导; ② 纳洛酮和BIC均可以部分减轻酒精对LTP诱导的抑制作用.结论内源性阿片系统和GABA能系统参与了酒精对LPP-CA3通路LTP诱导的调制.

关键词: 酒精, 长时程增强, 纳洛酮, 群体兴奋性突触后电位, 荷包牡丹碱, 穿通纤维

Abstract: Objective: To investigate the effect of alcohol on the induction of long-term potentiation(LTP)in lateral perforant pathway(LPP)-CA3 area of rats and its possible mechanism.Methods Taking the method of electro-physiology record in vivo, through micro-injection of alcohol,naloxone and bicuculline respectively to hippocampal CA3 area, using the slope coefficient chang of field excitory postsynaptic potentials(fEPSP) as index,to observe the LTP induction change caused by high frequency stimulation.Results (1) Single application of alcohol into CA3 area inhibited the induction of LTP in a dose-dependent manner.(2)Micro-injection of naloxone or bicuculline before administration of alcohol into CA3 area partially reduced the inhibitory effect of alcohol on the induction of LTP.ConclusionThe inhibition of alcohol on the induction of LTP in hippocampal CA3 area may be mediated by GABA energic system and endogenous opium system.

Key words: alcohol, long-term potentiation(LTP), naloxone, field excitory postsynaptic potentials, bicuculline, lateral perforant path(LPP)

中图分类号:

R338.11

杨荣,杨友华,陈晓青,黄丹. 酒精对大鼠外侧穿通纤维-CA3区LTP诱导的影响[J]. 江汉大学学报（自然科学版）, 2011, 39(2): 66-69.

YANG Rong, YANG You-hua, CHEN Xiao-qing, HUANG Dan. Effects of Alcohol on Induction of Long-term Potentiation in Lateral Perforant Path-CA3 Area of Rats[J]. Journal of Jianghan University(Natural Science Edition), 2011, 39(2): 66-69.

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