335例乙型肝炎患者血清HBV－DNA定量与乙肝病毒标志物的相关性分析

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (5): 93-95.

335例乙型肝炎患者血清HBV－DNA定量与乙肝病毒标志物的相关性分析

陈凤萍，沈云峰，吴瑶，张洪波

江汉大学附属医院检验科，湖北武汉430015

收稿日期:2012-08-21 出版日期:2012-10-20 发布日期:2013-11-07
作者简介:陈凤萍(1981—)，女，医师，硕士，研究方向:临床免疫学。及分子生物学。

Correlated Analysis of Serum HBV－DNA Level and HBV Markersin 335 Cases of Hepatitis B Patients

CHEN Feng－ping,SHEN Yun－feng,WU Yao,ZHANG Hong－bo

Clinical Lab,Affiliated Hospital,Jianghan University,Wuhan 430015,Hubei,China

Received:2012-08-21 Online:2012-10-20 Published:2013-11-07

摘要/Abstract

摘要： 目的探讨乙型肝炎患者血清HBV－DNA定量与乙肝病毒标志物的关系。方法对335例乙型肝炎患者分别用实时荧光定量聚合酶链反应(FQ－PCR)检测血清HBV－DNA含量，酶联免疫吸附法(ELISA)检测血清乙肝病毒标志物，并对结果进行统计学分析。结果大三阳组HBV－DNA阳性率及含量显著高于其他组(P＜0.01);小三阳组HBV－DNA阳性率及含量显著低于大三阳组(P＜0.01)，但与HBsAg、HBcAb阳性组比较差异无统计学意义(P＞0.05);HBeAg阳性组HBV－DNA阳性率显著高于HBeAg阴性组(P＜0.01)。结论只有将HBV－DNA定量检测和乙肝病毒标志物有机的结合，才能为乙型肝炎的临床感染、病毒复制、传染性强弱以及抗病毒药物的疗效观察提供可靠的判断依据。

关键词: HBV－DNA, 实时荧光定量聚合酶链反应(FQ－PCR), 乙肝病毒标志物

Abstract: ObjectiveTo study the association between HBV－DNA level and HBV markers in hepatitis B patients. MethodsA total of 335 hepatitis B patients were recruited in this study. Serum HBV－DNA level was quantified by fluorescence quantitative polymerase chain reaction(FQ－PCR)and serum HBV markers were detected by enzyme－linked immunosorbent assay(ELISA) separately. The association between HBV－DNA level and HBV markers was primarily investigated by statistical analysis. ResultsThe HBV－DNA positivity and level were significantly higher in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients than those in other groups(P ＜ 0. 01); The HBV－DNA positivity and level were significantly lower in HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients than those in HBsAg(＋)、HBeAg(＋)、HBcAb(＋) patients(P ＜ 0. 01), but the differences were no statistical significance between HBsAg(＋)、HBeAb(＋)、HBcAb(＋) patients and HBsAg(＋)、HBcAb(＋) patients(P ＞ 0. 05); HBV－DNA positivity was significantly higher in HbeAg(＋) patients than in HbeAg(－) patients(P ＜ 0. 01). ConclusionCombining quantitative detection of HBV－DNA with HBV markers, in order to provide a reliable basis for judging the hepatitis B infection, virus replication, infectious intensity and the effect of antiviral observations.

Key words: HBV－DNA, fluorescence quantitative polymerase chain reaction (FQ－PCR), HBV markers

中图分类号:

R512.620.4

陈凤萍，沈云峰，吴瑶，张洪波. 335例乙型肝炎患者血清HBV－DNA定量与乙肝病毒标志物的相关性分析[J]. 江汉大学学报（自然科学版）, 2012, 40(5): 93-95.

CHEN Feng－ping,SHEN Yun－feng,WU Yao,ZHANG Hong－bo. Correlated Analysis of Serum HBV－DNA Level and HBV Markersin 335 Cases of Hepatitis B Patients[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(5): 93-95.