丹参酮ⅡA促内皮细胞增殖作用研究

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (5): 90-92.

丹参酮ⅡA促内皮细胞增殖作用研究

凃乾¹，叶勇¹，杨丹丹²

1.江汉大学医学院，湖北武汉430056；
2.乳山市人民医院，山东威海264500

收稿日期:2012-08-12 出版日期:2012-10-20 发布日期:2013-11-07
作者简介:凃乾(1975—)，女，副教授，博士，研究方向:中医药机制研究

Effect of TanshinoneⅡA Promoting Endothelial Cells Proliferation

TU Qian¹,YE Yong¹,YANG Dan-dan²

1.School of Medicine,Jianghan University,Wuhan 430056,Hubei,China;
2.Renming Hospital of Rushan,Weihai 264500,Shandong,China

Received:2012-08-12 Online:2012-10-20 Published:2013-11-07

摘要/Abstract

摘要： 目的观察活血化瘀类中药丹参的活性成分—丹参酮ⅡA对内皮细胞增殖的影响、及其适宜作用浓度和时间，并初步探讨其作用机制。方法不同浓度丹参酮ⅡA作用于人脐静脉内皮细胞系(HUV－EC－C)不同时间，利用SRB方法测得其吸光度值(OD)，以确定各组内皮细胞增殖情况，并筛选适宜浓度和适宜时间点。结果丹参酮ⅡA具有显著的促人脐静脉内皮细胞(HUV－EC－C)增殖效应，其中终浓度6mg／L干预48h效应最为显著。结论丹参酮ⅡA能够显著促进内皮细胞增殖，提示其具有促血管生成作用。

关键词: 丹参酮ⅡA, 细胞增殖, 血管生成

Abstract: ObjectiveTo study the effects of tanshinoneⅡA promoting endothelial cells proliferation and investigate the proper dose and action time. MethodsRespectively investigates the situation of cell proliferation with SRB after different concentration tanshinoneⅡA incubating the human umbilical vein cells (HUV-EC-C) for 24 h and 48 h. ResultsTanshinoneⅡA has the obvious effect to promote cell proliferation of human umbilical vein cells(HUV-EC-C), the effect is most obvious under the conditions that the concentration is 6 mg/L and the intervening time is 48 h. ConclusionTanshinoneⅡA can promote endothelial cell proliferation which play an important role on angiogenesis.

Key words: tanshinoneⅡA, cell proliferation, angiogenesis

中图分类号:

R285
R972

凃乾，叶勇，杨丹丹. 丹参酮ⅡA促内皮细胞增殖作用研究[J]. 江汉大学学报（自然科学版）, 2012, 40(5): 90-92.

TU Qian,YE Yong,YANG Dan-dan. Effect of TanshinoneⅡA Promoting Endothelial Cells Proliferation[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(5): 90-92.