大花蕙兰快速繁殖体系的初步建立

江汉大学学报（自然科学版） ›› 2015, Vol. 43 ›› Issue (3): 248-252.

大花蕙兰快速繁殖体系的初步建立

胡燕梅¹，夏景波²，方中明^*3 ，郭云贵³，杨永会³

1. 江汉大学期刊社，湖北武汉 430056；2. 暨南大学生命科学技术学院，广东广州 510632；3. 武汉生物工程学院生物科学与技术学院，湖北武汉 430415

出版日期:2015-06-28 发布日期:2015-07-02
通讯作者: 方中明
作者简介:胡燕梅（1979—），女，副教授，硕士，研究方向：植物学及植物生物技术。
基金资助:
国家自然科学基金青年基金项目（31301250）；湖北省教育厅科学技术研究项目（B20094008）；武汉市高校科学研究项目（2008K079）

Preliminary Establishment of Rapid Propagation System of Cymbidium Hybridum

HU Yanmei¹， XIA Jinbo²， FANG Zhongming^*3， GUO Yungui³， YANG Yonghui³

1. Periodicals Press，Jianghan University， Wuhan 430056， Hubei， China；2. School of Life Science and Technology，Jinan University， Guanghzou 510632，Guangdong，China；3. Life Science and Technology College，Wuhan Bioengineering Institute， Wuhan 430415，Hubei，China

Online:2015-06-28 Published:2015-07-02

摘要/Abstract

摘要： 探讨了4种大花蕙兰外植体诱导原球茎形成的效果和不同激素配比对茎尖原球茎诱导及增殖与分化培养的影响。结果表明：诱导原球茎适宜外植体为茎尖，培养60 d后诱导率达到76. 0%。而其他3种外植体均不能有效诱导原球茎形成；其中 0. 5 mg/L 6-BA +0. 1 mg/L NAA 激素组合诱导茎尖原球茎形成率达到87. 8%；而茎尖原球茎在各激素组合处理中增殖率均能达到100%。原球茎在1. 5 mg/L KT +0. 5 mg/L IBA 激素组合中增殖与分化效果最好，增殖系数和分化系数分别达到5. 19和2. 80。

关键词: 大花蕙兰, 快速繁殖, 茎尖, 原球茎, 增殖与分化

Abstract: The formation effects of protocorm of cymbidium induced by four kinds of explants are discussed，as well as the influence of hormone proportion to proliferation and differentiation of protocorm induction. The experiment results showed the best explants for protocorm induction were stem tips，the inducing rate reached 76. 0% after 60 days culture. The three other explants could not effectively induce the protocorm. With 0. 5 mg/L 6-BA +0. 1 mg/L NAA treatment，the formation rate of protocorm induced by stem tip reached 87. 8%，and the proliferation rate of protocorm induced by stem tip with each hormone treatment all reached 100%，the proliferation and differentiation of protocorm were the best with the treatment of 1. 5 mg/L KT + 0. 5 mg/L IBA，the proliferation coefficient and the differentiation coefficient are 5. 19 and 2. 80， respectively.

Key words: cymbidium, rapid propagation, stem tip, protocorm, proliferation and differentiation

中图分类号:

S682.31

胡燕梅，夏景波，方中明，郭云贵，杨永会. 大花蕙兰快速繁殖体系的初步建立[J]. 江汉大学学报（自然科学版）, 2015, 43(3): 248-252.

HU Yanmei， XIA Jinbo， FANG Zhongming， GUO Yungui， YANG Yonghui. Preliminary Establishment of Rapid Propagation System of Cymbidium Hybridum[J]. Journal of Jianghan University(Natural Science Edition), 2015, 43(3): 248-252.