外源激素对甜叶菊新品种‘惠农5 号’试管苗增殖及生根的影响

doi:10.16389/j.cnki.cn42-1737/n.2020.06.009

江汉大学学报（自然科学版） ›› 2020, Vol. 48 ›› Issue (6): 65-71.doi: 10.16389/j.cnki.cn42-1737/n.2020.06.009

外源激素对甜叶菊新品种‘惠农5 号’试管苗增殖及生根的影响

陈志英，李文阳，崔广荣^*

安徽科技学院农学院，安徽凤阳 233100

出版日期:2020-12-28 发布日期:2020-12-18
作者简介:陈志英（1996— ），女，硕士生，研究方向：作物种子发芽生理。
基金资助:
CUI Guangrong

Effects of Exogenous Hormones on Proliferation and Rooting of a New Stevia Cultiver ′huinong No. 5′ in vitro

CHEN Zhiying，LI Wenyang，CUI Guangrong^*

College of Agronomy，Anhui Science and Technology University，Fengyang 233100，Anhui，China

Online:2020-12-28 Published:2020-12-18
Contact: 崔广荣

摘要/Abstract

摘要： 本试验以MS 作为基本培养基，以‘ 惠农5 号’甜叶菊试管苗茎段为实验材料，研究了6-BA、KT、Ad 3 种外源激素的6 个浓度的溶液与0. 1 mg/L NAA 处理组合对试管苗增殖及生根的影响。试验结果表明，添加适宜浓度的外源激素作用效果是很明显的，其中适用于甜叶菊试管苗的继代培养形成的培养基为MS + 6-BA 1. 0 mg/L + NAA 0. 1 mg/L + 糖30 g/L + 琼脂粉5. 5 g/L；适合甜叶菊试管苗增殖的培养基为MS + 6-BA 2. 0 mg/L + NAA 0. 1 mg/L + 糖30 g/L + 琼脂粉5. 5 g/L，增殖系数可达3. 50；试管苗最佳生根培养基为1/2 MS + NAA 0. 1 mg/L + 糖20 g/L + 琼脂粉5. 5 g/L。不同外源激素种类、不同浓度及不同的处理组合对甜叶菊试管苗增殖及生根的影响不同。

关键词: 甜叶菊, 外源激素, 增殖, 生根

Abstract: In this experiment，MS was used as the basic medium and the stem segments of ′huinong No. 5′ stevia test-tube seedlings were used as experimental materials. The effects of the treatment combination of 6-BA，KT，Ad three exogenous hormone solutions at 6 concentrations and 0. 1 mg/L NAA on the proliferation and rooting of test-tube seedlings were studied. The results showed that the effects of adding an appropriate concentration of exogenous hormone were obvious. The medium suitable for the secondary culture of stevia test-tube seedlings was MS + 6-BA 1. 0 mg/L + NAA 0. 1 mg/L + sugar 30 g/L + AGAR powder 5. 5 g/L. The medium suitable for the proliferation of stevia test-tube seedlings was MS + 6-BA 2. 0 mg/L + NAA 0. 1 mg/L + sugar 30 g/L + AGAR powder 5. 5 g/L，the proliferation coefficient can reach 3. 50. The optimal rooting medium for test-tube seedlings was 1/2 MS + NAA 0. 1 mg/L + sugar 20 g/L + AGAR powder 5. 5 g/L. Different kinds of exogenous hormones，different concentrations and different combinations of treatments have different effects on the proliferation and rooting of stevia test-tube seedlings.

Key words: stevia, exogenous hormones, proliferation, rooting

中图分类号:

S513

陈志英，李文阳，崔广荣. 外源激素对甜叶菊新品种‘惠农5 号’试管苗增殖及生根的影响[J]. 江汉大学学报（自然科学版）, 2020, 48(6): 65-71.

CHEN Zhiying，LI Wenyang，CUI Guangrong. Effects of Exogenous Hormones on Proliferation and Rooting of a New Stevia Cultiver ′huinong No. 5′ in vitro[J]. Journal of Jianghan University (Natural Science Edition), 2020, 48(6): 65-71.

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外源激素对甜叶菊新品种‘惠农5 号’试管苗增殖及生根的影响

Effects of Exogenous Hormones on Proliferation and Rooting of a New Stevia Cultiver ′huinong No. 5′ in vitro

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