CTMAB催化增敏分光光度法测定食品中的己烯雌酚

江汉大学学报（自然科学版） ›› 2013, Vol. 41 ›› Issue (6): 27-30.

CTMAB催化增敏分光光度法测定食品中的己烯雌酚

陈荷莲，黄璐，郑琦^*

江汉大学化学与环境工程学院，湖北武汉 430056

出版日期:2013-12-12 发布日期:2014-01-09
通讯作者: 郑琦
作者简介:陈荷莲（1968—），女，讲师，研究方向：有机合成及分析。
基金资助:
湖北省教育厅科学技术研究项目（B2013146）；湖北省科技厅项目（2011CDC130）；武汉市科技局项目（201220837304-3）

Determination of Diethylstilbestrol in Food by Catalytic and Sensitive Spectrophotometry with CTMAB

CHEN He-lian，HUANG Lu，ZHENG Qi

School of Chemistry and Environmental Engineering， Jianghan University，Wuhan 430056，Hubei，China

Online:2013-12-12 Published:2014-01-09
Contact: ZHENG Qi

摘要/Abstract

摘要： 利用CTMAB（三甲基十六烷基溴化铵）具有相转移催化和增敏络合体系等特性，建立了用可见分光光度法测定己烯雌酚（DES）的方法。研究表明，DES能将 Fe（Ⅲ）还原成 Fe（Ⅱ），Fe（Ⅱ）与 K₃［Fe（CN）₆］在CTMAB存在的环境下形成稳定的多元胶束络合体系，其最大吸收波长在736 nm处。DES的质量浓度在0.0325 6～0.652 1 ug/mL范围内与吸光度呈良好线性关系，线性回归方程 A = 0.002 7 + 0.399 0ρ（μg/mL），相关系数R²= 0.999 1，摩尔吸光系数 ε = 5.43×105（L·mol^-1·cm^-1），检出限1.35 μg/mL，相对标准偏差为0.89%，方法用于测定奶粉中DES含量，回收率为89.3%～101.6%。

关键词: 己烯雌酚（DES）, 可见分光光度法, CTMAB

Abstract: A catalytic and sensitive spectrophotometric method for the determination of diethylstilbestrol （DES）with CTMAB has been developed. The method is based on the phase transfer catalysis and sensitive complex ation characteristic of CTMAB. The study showed that Fe（Ⅲ）was reduced to Fe（Ⅱ）by DES and a stable multi-micelle complex was formed by Fe（Ⅱ）and K₃［Fe（CN）₆ ］in the existence of CTMAB，the maximum absorption is at at 736 nm. Lambert-Beer law was well obeyed at DES concentrations ranging from 0. 032 56 to 0. 652 1 μg/mL，regression equation was A = 0. 002 7+0. 399 0 ρ，correlation coefficient R² was 0. 999 1，detection limit was 1. 35 μg/mL and the molar absorption coefficient was ε = 5. 43 × 105（L·mol^-1·cm^-1）；the relative standard deviation was 0. 89%. The proposed method was used in the determination of DES in powdered milk and the recovery was 89.3% ～ 101.6%.

Key words: diethylstilbestrol（DES）, visible spectrophotometry, CTMAB

中图分类号:

陈荷莲，黄璐，郑琦. CTMAB催化增敏分光光度法测定食品中的己烯雌酚[J]. 江汉大学学报（自然科学版）, 2013, 41(6): 27-30.

CHEN He-lian，HUANG Lu，ZHENG Qi. Determination of Diethylstilbestrol in Food by Catalytic and Sensitive Spectrophotometry with CTMAB[J]. Journal of Jianghan University(Natural Science Edition), 2013, 41(6): 27-30.