江汉大学学报(自然科学版) ›› 2013, Vol. 41 ›› Issue (1): 93-95.

• 医学 • 上一篇    下一篇

凝胶剂中脂溶性茶多酚的含量测定

田永强1,2,张涛2*,陆茜3,Stephen Hsu4,艾永循2   

  1. 1.湖北中医药大学,湖北 武汉 430065; 2. 江汉大学 医学院,湖北 武汉 430056;3.江汉大学 化学与环境工程学院,湖北 武汉 430056;4.Georgia Health Science University,Augusta GA US 30912-1126
  • 出版日期:2013-02-12 发布日期:2013-12-27
  • 通讯作者: 张涛
  • 作者简介:田永强(1986—),男,硕士生,研究方向:中药及天然产物的化学与药理。
  • 基金资助:
    武汉市科技局江汉大学创新基地建设项目(201250499145-29)

Determination of Content of Lipo Soluble Tea Polyphenols in Gel

TIAN Yong-qiang1,2,ZHANG Tao2,LU Qian3,Stephen Hsu4,AI Yong-xun2   

  1. 1.Hubei University of Traditional Chinese Medicine,Wuhan 430065,Hubei,China;2.School of Medicine,Jianghan University, Wuhan 430056,Hubei,China;3. School of Chemistry and Environmental Engineering,Jianghan University,Wuhan 430056,Hubei,China;4.Georgia Health Science University, Augusta GA US 30912-1126
  • Online:2013-02-12 Published:2013-12-27
  • Contact: ZHANG Tao

摘要: 目的:建立凝胶剂中脂溶性茶多酚的含量测定方法,为制定脂溶性茶多酚凝胶剂质量控制标准提供依据。方法:以无水乙醇为提取溶剂,酒石酸亚铁为显色剂,采用比色法,在540 nm处测定含量。结果:脂溶性茶多酚在0~250 mg·(100 mL)-1 浓度范围内线性关系良好(R2= 0.9991),平均回收率为102.80%,相对标准偏差为0.93%(n = 9)。结论:本法简单、精确、重复性好,可用于凝胶剂中脂溶性茶多酚的含量测定。

关键词: 脂溶性茶多酚, 凝胶剂, 比色法, 含量测定

Abstract: Objective:To establish the method of determining the content of lipo soluble tea polyphenols in gel for quality control standard. Methods:A colorimetric method was established for the determination of lipo soluble tea polyphenols in gel at 540 nm with ethanol as the extracting solvent and tartaric acid ferrous as the coloring reagent. Results:The linear relationship of lipo soluble tea polyphenols is good in the range of 0 to 250 mg· (100 mL) -1(R2=0.999 1)and the average recov?ery is 102. 80%,the relative standard deviation(RSD)is 0.93%(n=9) . Conclusion:This method is simple,accurate,repeatability good and can be used for determining the content of lipo soluble tea polyphenols in gel.

Key words: lipo soluble tea polyphenols, gel, colorimetry, content determination

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