C60/Cu2＋影响质粒DNA构象的复合效应研究

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (5): 80-84.

C60/Cu2＋影响质粒DNA构象的复合效应研究

李佳¹，林燕²，李准洁³，李正¹，梁勇¹

1.江汉大学医学院，湖北武汉430056；
2.华中农业大学资源与环境学院，湖北武汉430070；
3.江汉大学生命科学学院，湖北武汉430056

收稿日期:2012-07-06 出版日期:2012-10-20 发布日期:2013-11-07
通讯作者: 梁勇(1976—)，男，副教授，博士，研究方向:生态毒理学。E－mail:ly76＠263.net
作者简介:李佳(1981—)，女，讲师，硕士，研究方向:生态毒理学。
基金资助:
国家自然科学基金(20907017,20890112

Combined Effect Study of C60／Cu2＋ Affecting Plasmid DNA Conformation

LI Jia¹,LIN Yan²,LI Zhun－jie³,LI Zheng¹,LIANG Yong¹

1. School of Medicine,Jianghan University,Wuhan 430056,Hubei,China;
2. College of Recourses and Environment,Huazhong Agricultural University,Wuhan 430074,China;
3. College of Life Scicence,Jianghan University,Wuhan 430056,Hubei,China

Received:2012-07-06 Online:2012-10-20 Published:2013-11-07

摘要/Abstract

摘要： 碳纳米材料的毒性效应日益受到广泛关注，通过比较研究多种非光催化条件下，富勒烯(C60)／Cu2＋共存时对质粒DNA构象影响的复合作用，借以评估碳纳米材料的毒性效应。实验结果显示在非光催化条件下，C60／Cu2＋可显著诱导DNA构象从超螺旋DNA转变成开环DNA，并且这种剪切效应随C60和Cu2＋浓度的升高而增强;断裂后的DNA片段可重新被T4DNA连接酶连接成环状DNA，这说明C60／Cu2＋的剪切位点为磷酸二酯键。另外，多种活性氧捕获剂均不能有效消除C60／Cu2＋对DNA的剪切作用，这与Cu2＋在DNA裂解中起主要作用相关。上述研究结果表明在非光催化条件下，C60／Cu2＋可有效诱导DNA断裂，具有明显的复合效应，其潜在的基因毒性值得关注。

关键词: Cu2＋, C60, T4DNA连接酶, ROS

Abstract: The toxic effects of carbon nanoparticles have been increasingly gaining attention. In this research, the combined effect of C60／Cu2＋ on DNA conformation was comparatively studied by altering various reaction conditions, to assess the biological toxicity of carbon nanoparticles. The results showed that without photoexcitation, C60／Cu2＋ could significantly induce the conversion of supercoiled DNA into nicked DNA, and this DNA cleavage effect was enhanced as the concentration of Cu2＋ and C60 increased. Moreover, the cleaved DNA could be reconnected to circular DNA by T4 DNA ligase, which indicated that the phosphodiester bond was the splicing site of C60／Cu2＋. In addition, various ROS savengers could not reduce the DNA cleavage effect of C60／Cu2＋, which was relate to the feature that Cu2＋ played a pivotal role in DNA cleavage. Taken together, these results demonstrated that C60／Cu2＋ could significantly induce the DNA cleavage without photoexcitation, which exhibited obvious combined effect. Further consideration should be given in assessment of their potential genotoxicity.

Key words: Cu2＋, C60, T4 DNA ligase, ROS

中图分类号:

Q555.4

李佳，林燕，李准洁，李正，梁勇. C60/Cu2＋影响质粒DNA构象的复合效应研究[J]. 江汉大学学报（自然科学版）, 2012, 40(5): 80-84.

LI Jia,LIN Yan,LI Zhun－jie,LI Zheng,LIANG Yong. Combined Effect Study of C60／Cu2＋ Affecting Plasmid DNA Conformation[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(5): 80-84.

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