cAMP依赖蛋白激酶(PKA)磷酸化Nef蛋白对HIV复制的影响

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (3): 77-82.

cAMP依赖蛋白激酶(PKA)磷酸化Nef蛋白对HIV复制的影响

李培林¹，²

1.哈佛大学医学院医学系，波士顿MA02115；
2.加州大学旧金山分校医学院医学系，旧金山CA94121

收稿日期:2012-03-12 出版日期:2012-06-20 发布日期:2013-11-07
作者简介:李培林(1958—)，男，副研究员，硕士，研究方向:分子免疫学。。

2012Impact of HIV Replication by PKA Phosphorylation of HIV NefLEE P.

1. Department of Medicine,Harvard Medical School,Boston MA 02115,USA;
2. Department of Medicine,University of California San Francisco,San Francisco CA 94121,USA

Received:2012-03-12 Online:2012-06-20 Published:2013-11-07

摘要/Abstract

摘要： 目的研究NefPKA磷酸化位点对HIV复制的影响。方法在全长HIVNL4-3中，通过将NefSer9突变为丙氨酸，构建一个单突变HIVDNA。接着用野生型HIV或单突变型HIV产生病毒，然后用病毒去感染外周血单核细胞(PBMC)。结果与亲代病毒比较，单突变Ser9为丙氨酸的病毒下调了HIV在静息PBMC中的复制。结论该突变在消除静息原代细胞中Nef对HIV复制的影响起重要作用。研究结果显示，PKA对Nef磷酸化是静息细胞中病毒生命周期的重要步骤。

关键词: HIV, Nef蛋白, 磷酸化, 蛋白激酶PKA

Abstract: ObjectiveTo study impact of Nef PKA phosphorylation site on HIV replication. MethodsTo mutate Nef Ser9 to alanine in the context of full－length HIVNL4－3 to make a single mutant of HIV DNA, then make viral stock by using either wild－type HIV or the single mutant HIV. Finally using these viral stocks to infect PBMCs. ResultsVirus made from single mutant of Ser9 to alanine lowered HIV replication in resting PBMCs compared to parental virus. ConclusionThis mutation plays a major role in abrogating the Nef effect on HIV replication in unstimulated primary cells. This result suggests that Nef phosphorylation by PKA is an important step in the viral life cycle in resting cells.

Key words: HIV, Nef, phosphorylation, protein kinase A

中图分类号:

R512.91

李培林，. cAMP依赖蛋白激酶(PKA)磷酸化Nef蛋白对HIV复制的影响[J]. 江汉大学学报（自然科学版）, 2012, 40(3): 77-82.

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