罗非鱼鳞胶原蛋白提取工艺研究

江汉大学学报（自然科学版） ›› 2012, Vol. 40 ›› Issue (3): 105-107.

罗非鱼鳞胶原蛋白提取工艺研究

孙宪迅，孙齐英，韩雪，周理红

江汉大学生命科学学院，湖北武汉430056

收稿日期:2012-03-20 出版日期:2012-06-20 发布日期:2013-11-07
作者简介:孙宪迅(1976—)，男，讲师，博士生，研究方向:生物资源开发与利用
基金资助:
湖北省教育厅资助项目(B20083401)；武汉市教育局资助项目(2008K005)；武汉市科技局资助项目(2010－6－5)

Research on Technology of Extraction Collagen from Tilapia Fish Scales

SUN Xian-xun,SUN Qi-ying,HAN Xue,ZHOU Li-hong

School of Life Sciences,Jianghan University,Wuhan 430056,Hubei,China

Received:2012-03-20 Online:2012-06-20 Published:2013-11-07

摘要/Abstract

摘要： 研究用胃蛋白酶从罗非鱼鳞中提取胶原蛋白的工艺。根据测定水解液中羟脯氨酸(HYP)的含量计算胶原蛋白提取率，采用单因素和正交试验确定胃蛋白酶水解鱼鳞制备胶原蛋白的适宜酶解条件。结果表明，采用底物质量分数7％，胃蛋白酶酶解罗非鱼鳞制备胶原蛋白的适宜温度、pH、加酶量、时间分别为65℃、2.0、2％、2h。在适宜工艺条件下，胶原蛋白提取率可达94.24％。

关键词: 罗非鱼鳞, 胶原蛋白, 胃蛋白酶

Abstract: The technology of extraction collagen from tilapia fish scales through pepsin hydrolysis has been studied. According to hydrolysate concentration of hydroxyproline(HYP), the single factor and orthogonal experiment was determined the best enzymatic hydrolysis condition. The optimum condition of temperature, pH, enzyme concentration and hydrolyzing time for the extraction is 65℃、 pH 2.0、 2％、 2 h. The extraction rate of collagen from tilapia fish scales was 94.24％ under this optimal condition.

Key words: tilapia fish scale, collagen, pepsin

中图分类号:

TS254.9

孙宪迅，孙齐英，韩雪，周理红. 罗非鱼鳞胶原蛋白提取工艺研究[J]. 江汉大学学报（自然科学版）, 2012, 40(3): 105-107.

SUN Xian-xun,SUN Qi-ying,HAN Xue,ZHOU Li-hong. Research on Technology of Extraction Collagen from Tilapia Fish Scales[J]. Journal of Jianghan University(Natural Science Edition), 2012, 40(3): 105-107.